解剖学报

›› 2012, Vol. 43 ›› Issue (6): 818-821.doi: 10.3969/j.issn.0529-1356.2012.06.019

• 组织学胚胎学发育生物学 • 上一篇    下一篇

血管内皮生长因子和血小板反应蛋白-1在大鼠胸主动脉瘤中的表达 

范军1; 柏树令1* ; 阎亚伟1; 李想2; 田晓红1; 佟浩1   

  1. 1.中国医科大学基础医学院组织工程学教研室，沈阳 110001; 2.中国医科大学基础医学院细胞生物学教研室，沈阳 110001
  • 收稿日期:2012-05-25 修回日期:2012-07-01 出版日期:2012-12-06
  • 通讯作者: 柏树令

Expression of vascular endothelial growth factor and Thrombospondin-1 in rat thoracic aortic aneurysm 

  1. 1.Department of Tissue Engineering, College of Basic Medical Sciences, China Medical University, Shenyang 110001， China; 2.Department of Cell Biology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China
  • Received:2012-05-25 Revised:2012-07-01 Online:2012-12-06
  • Contact: BAI Shu-ling

摘要： 目的 探讨血管内皮生长因子（VEGF）及血小板反应蛋白-1（TSP-1）在实验性大鼠胸主动脉瘤中的表达及其意义。方法 30只成年雄性Wistar大鼠随机分为对照组和手术组，每组15只。采用氯化钙（CaClSUB>2/SUB>）诱导法制备胸主动脉瘤模型,于术后4周取外敷CaCl2段胸主动脉。HE染色及地衣红染色观察胸主动脉组织学改变；免疫组织化学和免疫印迹法检测动脉瘤壁VEGF和TSP-1的表达。结果 免

关键词: 血管内皮生长因子, 血小板反应蛋白-1, 胸主动脉瘤, 免疫组织化学, 免疫印迹法, 大鼠

Abstract: Objective To investigate the expression and significance of vascular endothelial growth factor（VEGF）and thrombospondin-1（TSP-1）in the experimental rat thoracic aortic aneurysm. Methods Thirty adult male Wistar rats were randomly divided into two groups: sham group and operated group. The thoracic aortic aneurysm model was induced by CaClSUB>2/SUB> . The thoracic aortic segments were collected at 4 weeks after surgery. HE staining and Orcein staining were used to observe histological changes in the thoracic aorta. Immunohistochemistry and Western blotting were performed to detect the expressions of VEGF and TSP-1 in the wall of aneurysm. Results Immunohistochemistry staining results showed that VEGF was mainly located in the tunica media and tunica adventitia in the aortic aneurysm and there was few positive staining in the control group. TSP-1 was located in the normal aorta but the positive staining in the aneurysm was weak. Western blotting results indicated that the expression level of VEGF in the aortic aneurysm was stronger than the control, while the expression level of TSP-1 was weaker than the control. Conclusion Both TSP-1 and VEGF participate in the pathogenesis of thoracic aortic aneurysm. They may be the key factors in the new vessel formation of aortic aneury

Key words: Vascular endothelial growth factor, Thrombospondin-1, Thoracic aortic aneurysm, Immunohistochemistry, Western blotting, Rat

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