转录因子GATA4的表达模式与小鼠胚胎心动脉端发育的关系

doi:10.3969/j.issn.0529-1356.2013.02.021

解剖学报 ›› 2013, Vol. 44 ›› Issue (2 ): 253-258.doi: 10.3969/j.issn.0529-1356.2013.02.021

• 组织学胚胎学发育生物学 • 上一篇下一篇

转录因子GATA4的表达模式与小鼠胚胎心动脉端发育的关系

蔡玉瑾¹ 景雅^1* 乔爱秀² 杨艳萍¹ 李海荣¹ 崔慧林¹ 张涛¹

1.山西医科大学组织学与胚胎学教研室; 2.病理学教研室，太原 030001

收稿日期:2012-04-23 修回日期:2012-05-24 出版日期:2013-04-06 发布日期:2013-04-06
通讯作者: 景雅 E-mail:jingya66@hotmail.com
基金资助:
省自然科学基金资助项目;国家自然科学基金资助项目

Association of transcriptional factor GATA4 expression patterns with arterial pole development of embryonic mouse heart

CAI Yu-jin¹JING Ya ^1* QIAO Ai-xiu² YANG Yan-ping¹ LI Hai-rong¹ CUI Hui-lin¹ ZHANG Tao¹

1. Department of Histology and Embryology; 2. Department of Pathology, Shanxi Medical University, Taiyuan 030001, China

Received:2012-04-23 Revised:2012-05-24 Online:2013-04-06 Published:2013-04-06

摘要/Abstract

摘要：

目的探讨GATA4在生心区及前肠内胚层的时空表达与小鼠胚胎心动脉端发育的形态学关系。方法取胚龄7.5~13d小鼠胚胎各3个，连续石蜡切片，用抗GATA4、抗Nkx2.5、抗胰岛素增强子结合蛋白、抗α-平滑肌肌动蛋白和抗心肌肌球蛋白重链抗体，进行免疫组织化学或免疫荧光染色；Western blotting检测GATA4与磷酸化组蛋白H3(pHH3)在胚龄11~14d小鼠胚胎心的表达。结果小鼠胚胎7.5d，GATA4和Nkx2.5同时表达于生心板，胚龄8.5~10d进一步表达于心包腔背侧壁第二生心区的脏壁中胚层，胚龄11~13d，两者的表达从流出道与心包腔背侧壁交界处降至主肺动脉根部。胚龄第11~13天，GATA4见于前肠内胚层。GATA4与pHH3的表达高峰见于胚龄11~13d，胚龄14d显著减少。结论小鼠胚胎发育早期，GATA4与Nkx2.5在生心区的表达模式相同，GATA4可能促进心肌细胞增生，前肠内胚层的GATA4可能参与第二生心区的发育。

关键词: GATA4 , 生心区 , 内胚层 , 免疫组织化学 , 免疫印迹法 , 小鼠

Abstract:

Objective To explore the relationship of the expression of transcriptional factor GATA4 in the heart-forming field and the foregut endoderm with the morphological development of the arterial pole of embryonic mouse heart.Methods Serial sections of mouse embryos on embryonic day(ED)7.5 to ED13, three embryos from each embryonic day, were stained immunohistochemically or immunofluorescently with antibodies against GATA4, Nkx2.5, insulin gene enhancer binding potein (ISL-1), α-smooth muscle actin and myosin heavy chain. The expression of GATA4 and phosphorylated histone H3(pHH3) were measured with Western blotting in the embryonic mouse hearts from ED11 to ED14. Results At ED7.5, positive cells for both transcription factors GATA4 and Nkx2.5 were found in the cardiogenic plate simultaneously. From ED8.5 to ED10, further expression of GATA4 and Nkx2.5 were detected in the splanchnic mesoderm of the dorsal wall of the pericardial cavity that constituted the second heart field(SHF). From ED11 to ED13, GATA4 and Nkx2.5 expressions were found descending from the reflection of dorsal pericardial wall with the distal pole of outflow tract to the base of the ascending aorta and pulmonary trunk. GATA4 expression was also detected in the foregut endoderm of ED11 to ED13 embryos. GATA4 and pHH3 expression in the embryonic mouse hearts reached the highest level between ED11 and ED13. Conclusion Transcription factor GATA4 shows the same expression pattern as that of Nkx2.5 during the early development of the embryonic mouse heart. Higher expression of GATA4 during crucial stages of heart morphogenesis suggests that GATA4 may play an important role in the proliferation of myocardial cells. The GATA4 expression in the foregut endoderm may be involved in the development of the SHF.

Key words: GATA4 , Heart-forming field , Endoderm , Immunohistochemistry , Western blotting , Mouse

中图分类号:

Q132

蔡玉瑾景雅* 乔爱秀杨艳萍李海荣崔慧林张涛 . 转录因子GATA4的表达模式与小鼠胚胎心动脉端发育的关系[J]. 解剖学报, 2013, 44(2 ): 253-258.

CAI Yu-jin JING Ya* QIAO Ai-xiu YANG Yan-ping LI Hai-rong CUI Hui-lin ZHANG Tao. Association of transcriptional factor GATA4 expression patterns with arterial pole development of embryonic mouse heart[J]. AAS, 2013, 44(2 ): 253-258.

参考文献

［1］ Sun Y, Liang X, Najafi N, et al. Islet1 is expressed in distinct cardiovascular lineages, including pacemaker and coronary vascular cells［J］. Dev Biol, 2007, 304(1):286-296.

［2］Takeuchi JK，Bruneau BG. Directed transdifferentiation of mouse mesoderm to heart tissue by defined factors［J］. Nature, 2009, 459(7247):708-711. 

［3］Pikkarainen S, Tokola H, Kerkel R, et al. GATA transcription factors in the developing and adult heart［J］. Cardiovasc Res, 2004, 63(2):196-207.

［4］Waldo KL,Kumiski DH,Wallis KT,et al.Conotruncal myocardium arises from a secondary heart field［J］. Development, 2001, 128(16):3179-3188.

［5］Kasahara H, Bartunkova S, Schinke M, et al. Cardiac and extracardiac expression of Csx/Nkx2.5 homeodomain protein［J］. Circ Res, 1998,

82(9):936-946.

［6］Cai CL, Liang X, Shi Y, et al. Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart［J］. Dev Cell, 2003, 5(6):877-889.

［7］ Molkentin JD, Lin Q, Duncan SA, et al. Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis［J］. Genes Dev, 1997, 11(8):1061-1072.

［8］Zeisberg EM, Ma Q, Juraszek AL, et al. Morphogenesis of the right ventricle requires myocardial expression of Gata4［J］. J Clin Invest, 2005, 115(6):1522-1531.

［9］ Dodou E, Verzi MP, Anderson JP, et al. Mef 2c is a direct transcriptional target of ISL1 and GATA factors in the anterior heart field during mouse embryonic development［J］. Development, 2004, 131(16):3931-3942.

［10］Rivera-Feliciano J, Lee KH, Kong SW, et al. Development of heart valves requires Gata4 expression in endothelialderived cells［J］. Development, 2006, 133(18):3607-3618.

［11］ Zhao R, Watt AJ, Battle MA, et al. Loss of both GATA4 and GATA6 blocks cardiac myocyte differentiation and results in acardia in mice［J］. Dev Biol, 2008, 317(2):614-619.

［12］ Holtzinger A, Rosenfeld GE, Evans T. Gata4 directs development of cardiac-inducing endoderm from ES cells［J］. Dev Biol, 2010, 337(1):63-73.

［13］Lough J, Sugi Y. Endoderm and heart development［J］. Dev Dyn, 2000, 217(4):327-342.

［14］ Wu ShSh, Jing Y, Yang YP, et al. Association of neural crest cells and ISL-1 positive cells with the development of the outflow tract of the mouse embryonic heart［J］.Acta Anatomica Sinica, 2011, 42(5): 684-689.(in Chinese)

武姗姗, 景雅, 杨艳萍, 等. 神经嵴细胞和胰岛因子1阳性细胞与小鼠胚胎心流出道发育的关系［J］. 解剖学报, 2011, 42(5): 684-689. 

［15］ Ma Q, Zhou B, Pu WT. Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity［J］. Dev Biol, 2008, 323(1):98-104.

［16］Xin M, Davis CA, Molkentin JD, et al. A threshold of GATA4 and GATA6 expression is required for cardiovascular development［J］. Proc Natl Acad Sci USA, 2006, 103(30):11189-11194.

［17］ Pu WT, Ishiwata T, Juraszek AL, et al. GATA4 is a dosage-sensitive regulator of cardiac morphogenesis［J］. Dev Biol, 2004, 275(1):235-244.

［18］ Holtzinger A, Evans T. GATA4 regulates the formation of multiple organs［J］. Development, 2005, 132(17):4005-4014. 

[1]	洪晓敏李三强崔钦奕郑润月杨孟利罗仁利李前辉 . 酒精性肝纤维化核因子κB信号通路与性别的差异 [J]. 解剖学报, 2024, 55(1): 55-61.
[2]	魏茜茜李超红赵晨露唐家萍刘玉珍. 大鼠颈上神经节中Ⅰ组代谢型谷氨酸受体表达及慢性间歇性低氧对其的影响 [J]. 解剖学报, 2024, 55(1): 3-9.
[3]	袁蕾杨智伟杨惠刘政海何洁万炜. 三七总皂苷抑制小鼠星形胶质细胞活化缓解完全弗氏佐剂所致炎性痛 [J]. 解剖学报, 2024, 55(1): 25-31.
[4]	马婷婷陈建红刘爱翠李海宁. 抑制lncRNA TUG1下调核苷酸结合寡聚结构域样受体蛋白1炎症小体在延缓阿尔茨海默病进展的作用 [J]. 解剖学报, 2024, 55(1): 32-42.
[5]	邹成功冯浩陈兵唐辉邵川孙谋杨荣何家全. 创伤性颅脑损伤中神经功能障碍与认知功能损伤的动态变化 [J]. 解剖学报, 2024, 55(1): 43-48.
[6]	吕海燕沈西雅赵富生朱梅. 松茸多糖对 1-甲基-4-苯基-吡啶离子诱导 PC12 细胞损伤的影响 [J]. 解剖学报, 2024, 55(1): 49-54.
[7]	郑丽平刘霞杜晓华吴亚娟刘珊珊 . 脑源性神经营养因子在牦牛与黄牛端脑不同区域的表达与分布 [J]. 解剖学报, 2024, 55(1): 10-16.
[8]	郝永明郭磊周程辉裴汉军李莉赵哲 . 改良腹主动脉缩窄法建立心肌肥厚模型[J]. 解剖学报, 2024, 55(1): 120-124.
[9]	许亚平余悦欣陈金福王柯柯郭志坤 . 高龄大鼠心室肌间质弹性纤维和胶原纤维的结构分布特征及其机制 [J]. 解剖学报, 2023, 54(6): 716-721.
[10]	刘丽平朱梦妮徐慧 . 白细胞介素6对脂多糖诱导所致类子痫前期大鼠有核红细胞的影响 [J]. 解剖学报, 2023, 54(6): 722-729.
[11]	王文娟丁雨桐苏昊任捷孙艳荣王涵菲陆嘉莉张林倩白钰秦丽华. 低雌激素状态下大鼠海马及纹状体Nogo-A的表达变化[J]. 解剖学报, 2023, 54(6): 620-627.
[12]	张琴杨俊霞蒋青松. 福辛普利对糖尿病视网膜病变小鼠血管紧张素转化酶2和血管内皮生长因子的影响[J]. 解剖学报, 2023, 54(6): 628-634.
[13]	陈子璇司丽娜舒薇菡张欣魏晨阳程露阳杨松鹤乔跃兵. 褪黑素调控下丘脑PI3K/Akt/mTOR信号延缓雌性小鼠青春期启动的机制 [J]. 解剖学报, 2023, 54(6): 644-651.
[14]	梁盼盼禹爱梅杜静寇文辉王欢欢宋爱霞. 基于c-Jun氨基末端激酶/p38丝裂原活化蛋白激酶信号通路探讨阿魏酸钠对偏头痛大鼠炎症反应的抑制作用[J]. 解剖学报, 2023, 54(6): 652-659.
[15]	徐颖刘斌郑兴何宗钊. 补体C3a受体在盲肠结扎穿孔致脓毒症大鼠认知障碍中的作用及机制 [J]. 解剖学报, 2023, 54(6): 668-675.

转录因子GATA4的表达模式与小鼠胚胎心动脉端发育的关系

Association of transcriptional factor GATA4 expression patterns with arterial pole development of embryonic mouse heart

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价