解剖学报 ›› 2013, Vol. 44 ›› Issue (3 ): 379-385.doi: 10.3969/j.issn.0529-1356.2013.03.018

• 组织学胚胎学发育生物学 • 上一篇    下一篇

啮齿类动物视网膜血管系统的发育

姚焕玲 王天仕 刘鼎 李小菲 刘静 邓锦波*   

  1. 河南大学生命科学学院神经生物研究所, 河南 开封 475004
  • 收稿日期:2012-05-07 修回日期:2012-08-29 出版日期:2013-06-06 发布日期:2013-07-16
  • 通讯作者: 邓锦波 E-mail:jinbo_deng@henu.edu.cn
  • 基金资助:

    国家自然科学基金资助项目

Development of the retinal vascular system of rodents

YAO Huan-ling  WANG Tian-shi  LIU Ding  LI Xiao-fei  LIU Jing  DENG Jin-bo*   

  1. Institute of Neurobiology, College of Life Science, He’nan University, He’nan Kaifeng 475004, China
  • Received:2012-05-07 Revised:2012-08-29 Online:2013-06-06 Published:2013-07-16

摘要:

目的 探讨啮齿类视网膜血管系统的发育。方法 选取生后不同年龄阶段的啮齿类动物各12只,用免疫组织化学染色、明胶墨汁灌流、
透射电镜显示其视网膜血管和血-视网膜屏障结构。结果 啮齿类视网膜血管系统是生后由视盘处开始,并以视乳头为中心呈放射状蔓延生长,
逐步覆盖整个视网膜,先形成视网膜内表面浅层血管网,继而伸入视网膜内核层的内、外侧边缘形成两个平行于浅层血管网的深层血管网;血
-视网膜屏障由幼稚内皮细胞、厚薄不均一的基膜层和多层较厚的胶质细胞终足成分构成,逐步发育为由内壁光滑连接紧密的内皮细胞、均一的
基膜、较薄的终足构成的成熟血-视网膜屏障。结论 啮齿类动物视网膜血管系统的发育成熟与视网膜细胞的分化成熟在时空上存在一致性;成
熟的血-视网膜屏障使视网膜具有一定的抗感染能力。

关键词: 啮齿类, 视网膜血管系统, 免疫组织化学, 透射电镜, 明胶墨汁灌流, 小鼠, 大鼠

Abstract:

Objective To study the development of the retinal vascular system in rodents. Methods Immunohistochemistry,
gelatin-ink perfusion, and transmission electron microscopy (TEM) were utilized to demonstrate structures of the retinal
vasculature and blood-retinal barrier in rodents of different ages(n=12). Results The rodent retinal vascular system developed
from the optic disc after birth and radiated out to cover the entire retina. The superficial vasculature formed on the retinal
inner surface, then the vasculature extended into the inner and outer edges of the retinal inner nuclear layer, forming two
deep-layer vasculature that was parallel to the superficial vasculature. The blood-retinal barrier was initially composed of
naive endothelial cells, a basal membrane of non-uniform thickness, and multiple thick layers of glial cells terminal feet,
eventually it developed into a mature blood-retinal barrier composed of a smooth endothelial layer, even basal membrane, and
relatively thin terminal feet. Conclusion The maturation of rodent retinal vasculature is spatially and temporally is consistent
with the function performance of retinal photosensitivity. The mature blood-retinal barrier endows certain anti-infectious
immunity to the retina.

Key words: Rodent, Retina blood vasculature, Immunohistochemistry, Transmission electron microscopy, Gelatin-ink perfusion, Mouse, Rat 