胰岛淀粉样多肽、葡萄糖转运蛋白2在海洛因戒断、脱毒和复吸大鼠胰岛β细胞中的表达

doi:10.3969/j.issn.0529-1356.2013.06.012

摘要/Abstract

摘要：

Objective To investigate the expression changes of islet amyloid polypeptiole(IAPP) and glucose transporter 2(GLUT2) in islet β cells during heroin withdrawal induced by naloxone, methadone detoxification treatment, and heroin relapse. Methods Male SD rats (n=32) were randomly divided into a normal control group (NCG, n=8) and an experiment group (EG, n=24). The EG was further divided into heroin withdrawal group (HWG), methadone detoxication group (MDG), and heroin relapse group (HRG). Pancreas was drawn from each group. Immunohistochemistry and image analysis were used to detect the expression of IAPP and GLUT2. Results As compared with the normal control, the immunostainning of IAPP and GLUT2 was stronger in β cells of islets from heroin withdrawal and relapse rats than that in controls; the numerical density on area of IAPP also increased; the mean gray values decreased in these two groups. No difference was found in the immunostainning, the mean gray values and the numerical density on area during detoxification treatment compared to controls. Conclusion During heroin withdrawal and relapse, the synthesis of IAPP and GLUT2 increased, while they became decreased in methadone detoxification. The changes of IAPP and GLUT2 were consistent and had same rhythm in three stages. IAPP and GLUT2 took part in energy metabolic process, which may be related to food intake and body weight, or protective function toβcells.

关键词: 胰岛淀粉样多肽, 葡萄糖转运蛋白2, 胰岛β细胞, 海洛因, 美沙酮, 复吸, 免疫组织化学, 大鼠

Abstract:

objects To investigate the expression changes of IAPP and GLUT2 in islet βcells during heroin withdrawal induced by naloxone, methadone detoxification treatment, and heroin relapse. Methods Male Sprague-Dawley rats were divided into normal control group (NCG) and experiment group (EG). The EG was further divided into heroin withdrawal group (HWG), methadone detoxication group (MDG), and heroin relapse group (HRG). Pancreas was drawn from each group. Immunohistochemistry and image analysis were used to detect the expression of IAPP and GLUT2. Results As compared with the normal control, the immunostainning of IAPP and GLUT2 turned express intensive inβcells of islets from heroin withdrawal and relapse rats than that in controls; the numerical density on area of IAPP also increased; the mean gray values decreased in these two groups. There is no difference were found in the immunostainning, the mean gray values and the numerical density on area during detoxification treatment compared to controls. Conclusion During heroin withdrawal and relapse, the expression increased. While in detoxification, they became decreased. The changes of IAPP and GLUT2 are consistent and had same rhythm in three stages. It showed that IAPP and GLUT2 take participate in energy metabolic process, , which may be related to food intake and body weight, or protective function toβcells.

Key words: Islet amyloid polypeptide, Glucose transporter 2, Islet &beta, cell, Heroin, Methadone, Relapse, Immunohistochemistry, Rat

韩晶梁文妹* 洪艳夏白娟胡赟李一欣. 胰岛淀粉样多肽、葡萄糖转运蛋白2在海洛因戒断、脱毒和复吸大鼠胰岛β细胞中的表达[J]. 解剖学报, 2013, 44(6 ): 795-799.

HAN Jing LIANG Wen-mei* HONG Yan XIA Bai-juan HU Yun LI Yi-xin. Expression of islet amyloid polypeptide and glucose transporter 2 in the islet β cell of rat during heroin withdrawal, detoxification and relapse[J]. AAS, 2013, 44(6 ): 795-799.

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