解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (6 ): 804-811.doi: 10.3969/j.issn.0529-1356.2013.06.014

• 组织学胚胎学发育生物学 • 上一篇    下一篇

小鼠胚胎呼吸内胚层形态发生与咽前间充质发育及流出道分隔的关系

李慧超1,3 景雅1* 师亮1 杨艳萍1 李海荣1 乔从进1 乔爱秀2 蔡玉瑾1   

  1. 1. 山西医科大学组织学与胚胎学教研室; 2. 山西医科大学病理学教研室，太原 030001;3. 长春医学高等专科学校解剖学教研室， 长春 130031
  • 收稿日期:2013-02-01 修回日期:2013-03-20 出版日期:2013-12-06 发布日期:2013-12-06
  • 通讯作者: 景雅 E-mail:jingya66@hotmail.com
  • 基金资助:

    山西医科大学大学生创新基金资助项目;省自然科学基金资助项目;其他(不属于以上基金类别的请自行输入下框);国家自然科学基金资助项目;国家留学基金委资助项目

Association of morphogenesis of pulmonary endoderm with development of prepharyngeal mesenchyme and outflow tract septation in mouse embryos

LI Hui-chao 1,3 JING Ya 1* SHI Liang1 YANG Yan-ping1 LI Hai-rong1 QIAO Cong-jin1 QIAO Ai-xiu2 CAI Yu-jin1   

  1. 1. Department of Histology and Embryology, Shanxi Medical University; 2. Department of Pathology, Shanxi Medical University, Taiyuan 030001, China; 3. Department of Anatomy, Changchun Medical College, Changchun 130031, China
  • Received:2013-02-01 Revised:2013-03-20 Online:2013-12-06 Published:2013-12-06
  • Contact: JING Ya E-mail:jingya66@hotmail.com

摘要：

目的 探讨呼吸内胚层与咽前间充质细胞发育的关系及对小鼠胚胎心流出道分隔的影响。方法 45只胚龄8~13d小鼠胚胎心连续石蜡切片，用抗胰岛因子1（ISL-1）、抗α-平滑肌肌动蛋白（α-SMA）、抗音猬因子（音速波状蛋白， Shh）、抗patched（Ptc1）、抗patched 2（Ptc2）、抗smoothened（Smo）及抗心肌肌球蛋白重链（MHC）抗体进行免疫组织化学及免疫荧光染色。 结果 胚龄8～9d，ISL-1阳性细胞分布在心包腔背侧壁及前肠两侧间充质，并延伸至原始心管动脉端，心管心肌显较强的Ptc1和Ptc2阳性表达。胚龄10~13d，呼吸内胚层向腹侧延伸，Ptc1和Ptc2呈较强表达，ISL-1阳性咽前间充质细胞围绕呼吸内胚层形成对称的特征性锥体形结构，经动脉囊背侧壁伸入动脉囊腔，形成主肺动脉隔。胚龄12d，主肺动脉隔ISL-1阳性表达基本消失，大部分细胞转变为α-SMA阳性细胞。 结论 呼吸内胚层的分化发育与咽前ISL-1阳性间充质细胞的发育聚集密切耦联。发育中的呼吸内胚层可能作为组织中心，通过Shh信号通路对ISL-1阳性细胞的聚集提供位置信息。呼吸内胚层的正常腹侧延伸不但可诱导ISL1阳性细胞的正常迁移和流出道的正常分隔，对流出道的正常形态发生及有效的肺循环建立起重要作用。

关键词: 呼吸内胚层, 胰岛因子1阳性细胞, 心流出道, 音猬因子, 免疫组织化学, 免疫荧光, 小鼠

Abstract:

Objective To explore the relationship of pulmonary endoderm with the development of prepharyngeal mesenchyme and the outflow tract septation of the mouse embryonic heart. Methods Both the immunohistochemical and immunofluorescent methods were used in the present studies in order to observe islet-1(ISL-1), α-smooth muscle actin(α-SMA), sonic hedgehog(Shh); patched(Ptc1), patched 2(Ptc2), smoothened(Smo) and myosin heavy chain(MHC) distribution in 45 mice embryos from embryonic day(ED)8 to embryonic day(ED)13. Results From ED8 to ED9, ISL-1 positive cells distributed in the splanchnic mesoderm of the pericardial cavity dorsal wall, bilateral mesenchyme to the foregut, and extended distally to the primary heart tube. Ptc1, Ptc2 positive cells were detected in the myocardium of the heart tube. During ED10 to ED13, developing pulmonary endoderm extended ventrally and expressed Ptc1, Ptc2 strongly. ISL-1 positive prepharyngeal mesenchyme surrounding the pulmonary endoderm formed a symmetrically distinctive cone-shaped structure, which projected into the aortic sac to form the primary aorta-pulmonary septum. At ED12, most of the cells in aorta-pulmonary septum turned to express α-SMA, only a few ISL-1 positive cells could be detected. Conclusion The differentiation and development of pulmonary endoderm are closely associated with typical aggregation of ISL-1 positive prepharyngeal mesenchyme. The developing pulmonary endoderm probably acts as an organizing center via sonic hedgehog signaling pathway to confer positional information on ISL-1 positive cells aggregation. The ventral extension of the pulmonary endoderm may not only induce the accumulation and migration of ISL-1 positive cells, but also play important roles in normal morphogenesis of the outflow tract and establishment of effective pulmonary circulation.

Key words: Pulmonary endoderm, Islet-1 positive cell, Outflow tract of the heart, Sonic hedgehog, Immunohistochemisty, Immunofluorescence, Mouse