解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (6 ): 861-864.doi: 10.3969/j.issn.0529-1356.2013.06.022

• 细胞和分子生物学 • 上一篇    下一篇

人早孕绒毛细胞滋养层细胞培养方法的改良

左彦珍2 张孔雁3 李玉红1* 许倩4 毛晓丹4 谭宇思4   

  1. 1. 承德医学院病理学教研室; 2. 药理学教研室; 3. 附属医院老年病科; 4.基础研究所， 河北 承德 067000
  • 收稿日期:2013-01-14 修回日期:2013-05-10 出版日期:2013-12-06 发布日期:2013-12-06
  • 通讯作者: 李玉红 E-mail:120950413@qq.com
  • 基金资助:

    省教育厅项目;省自然科学基金资助项目

Isolation and culture of first trimester human trophoblast cell

ZUO Yan-zhen2 ZHANG Kong-yan3 LI Yu-hong 1* XU Qian4 MAO Xiao-dan4 TAN Yu-si4   

  1. 1. Department of Pathology, Chengde Medical College; 2. Department of Pharmacology; 3. Department of Geriatrics, Affiliated Hospital of Chengde Medical College; 4. Institute of Basic Medical, Chengde Medical College, Hebei Chengde 067000, China）
     
  • Received:2013-01-14 Revised:2013-05-10 Online:2013-12-06 Published:2013-12-06
  • Contact: LI Yu-hong E-mail:120950413@qq.com

摘要：

目的 探索一种简便高纯度的早孕滋养细胞的体外培养方法。方法 采用完整早孕绒毛通过胰蛋白酶联合胶原酶消化法分离，Percoll梯度分离法提纯体外培养，应用光镜HE染色和免疫细胞化学法检测细胞纯度。 结果 CK-7表达阳性细胞数可以达到90%以上。结论 完整绒毛消化和Percoll纯化联合可在短期内获得高纯度、高产量的滋养细胞以供后期试验，方法更加简便。

关键词: 滋养细胞, 早孕, 原代细胞培养, Percoll分离, 人

Abstract:

Objective To research a simple method for purification of first trimester human trophoblast cell in vitro.Methods Whole first trimester human chorionic tissues were isolated with trypsin/collagenase. HE staining and immunocytochemistry methods were used to detect the tyophoblast cells purity. Results Cytokeratin-7 positive cells were more than 90%. Conclusion First trimester human Trophoblast cells can be obtained through the simple method and may offer enough cells for later experiments.

Key words: Tyophoblast cell, First trimester, Primary cell culture, Percoll gradient, Human