解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (1): 104-108.doi: 10.3969/j.issn.0529-1356.2014.01.020

• 组织学胚胎学发育生物学 • 上一篇    下一篇

睾酮对小鼠卵泡体外发育的影响

牟晓莉 刘涛 刘洪彬 高媛 高选 耿玲 游力 许成岩*   

  1. 1. 山东大学附属省立医院生殖医学中心； 2. 国家辅助生殖与优生工程技术研究中心；3. 生殖内分泌教育部重点实验室； 4. 山东省生殖医学重点实验室, 济南 250021
  • 收稿日期:2013-05-24 修回日期:2013-08-20 出版日期:2014-02-06 发布日期:2014-02-06
  • 通讯作者: 许成岩 E-mail:muxiaoli1987@163.com
  • 基金资助:

    国家自然科学基金;中国博士后科学基金;山东省博士后创新基金;山东省博士后创新基金

Effect of testosterone on the in vitro mouse follicle development

MU Xiao-li LIU Tao LIU Hong-bin GAO Yuan GAO Xuan GENG Ling YOU Li XU Cheng-yan*   

  1. 1. Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University;2. National Research Center for Assisted Reproductive Technology and Reproductive Genetics;3. The Key Laboratory for Reproductive Endocrinology of Ministry of Education;4. Shandong Provincial Key Laboratory of Reproductive Medicine, Ji’nan 250021, China
  • Received:2013-05-24 Revised:2013-08-20 Online:2014-02-06 Published:2014-02-06
  • Contact: XU Cheng-yan E-mail:muxiaoli1987@163.com

摘要：

目的 探讨高浓度睾酮对体外培养的小鼠卵泡生长发育的影响。方法 以出生后12日龄的ICR雌鼠体外培养卵泡为研究模型，将卵泡随机分为以下4个组，每组30个：正常对照组（control组）、10-4 mol/L睾酮处理组、10-5 mol/L睾酮处理组和10-6 mol/L睾酮处理组。将卵泡随机分为以下3个组，每组30个：正常对照组（control组）、睾酮处理组（T组，T=10-5 mol/L）和雄激素受体拮抗剂氟他胺预处理后再加睾酮处理组（F+T组F=10-5mol/L，T=10-5 mol/L）。在60mm无菌细胞培养皿上进行微滴培养，每天在倒置显微镜下观察卵泡轮廓，颗粒细胞增生情况，卵母细胞形态以及有无卵母细胞逸出等，并记录卵泡存活和退化的情况。 结果 10-4 mol/L睾酮能抑制卵泡的生长，而10-5mol/L睾酮能够促进颗粒细胞的增殖和卵泡的生长；同时，在卵泡生长前期，睾酮能刺激卵泡的发育，在后期则呈抑制作用，即卵泡发育停滞、退化或死亡。 结论 在初级和次级卵泡阶段，10-5mol/L睾酮能促进卵泡的发育，而在窦状卵泡阶段则抑制卵泡的发育。

关键词: 睾酮, 卵泡发育, 体外培养, 小鼠

Abstract:

Objective To investigate the effect of testosterone on the in vitro mouse follicle development. Methods We chose follicles of 12-day-old ICR female mice culturedin vitro as our research models. Follicles were divided into four groups randomly with thirty follicles in each group and were treated with different concentration testosterone: 0(control group), 10-4 mol/L,10-5 mol/L,10-6 mol/L. Follicles were divided into three groups randomly with thirty follicles in each group and were treated with different reagents: no treatment (control group), 10-5 mol/L testosterone(group T), 10-5 mol/L androgen antagonist flutamide pretreatment plus 10-5 mol/L testosterone(group F+T). Follicles were cultured on microdrop. We observed follicle outlines, granulosa cells proliferation and oocytes morphology under the inverted microscope every day, recorded follicle degradation. Results The development of follicles was inhibited in the group treated with 10-4 mol/L testosterone. While the granulosa cells proliferation and the development of follicles were promoted in the group treated with 10-5 mol/L testosterone. Meanwhile, testosterone stimulated the follicles development in the early stage of the follicular development, but it inhibited the follicles development in the late stage. Conclusion 10-5 mol/L testosterone can promote the development of primary follicles and secondary follicles, but it can inhibit the antral follicles development.

Key words: Testosterone, Follicle development, In vitro culture, Mouse