基质细胞衍生因子-1α和白细胞介素-1β诱导淋巴管内皮表型的作用

doi:10.3969/j.issn.0529-1356.2014.03.017

解剖学报 ›› 2014, Vol. 45 ›› Issue (3): 388-392.doi: 10.3969/j.issn.0529-1356.2014.03.017

• 组织学胚胎学发育生物学 • 上一篇下一篇

基质细胞衍生因子-1α和白细胞介素-1β诱导淋巴管内皮表型的作用

索宁¹王雪颖²杨春林³周辉³李菲¹张宗璞¹万芳竹¹田铧^1*

1. 山东大学医学院解剖学与组织学胚胎学研究所，济南 250012; 2.山东省郯城县第一人民医院肿瘤科，山东临沂 276199; 3.山东大学技术支持科，济南 250012

收稿日期:2013-11-01 修回日期:2014-01-06 出版日期:2014-06-06 发布日期:2014-06-06
通讯作者: 田铧 E-mail:sduth@163.com
基金资助:
山东省自然科学基金;山东省医药卫生科技项目

Effect of stromal cell derived factor-1α and interleukin-1β on inducing vascular endothelial cells expressing lymphatic phenotype

SUO Ning¹WANG Xue-ying² YANG Chun-lin¹ ZHOU Hui³ LI Fei¹ ZHANG Zong-pu¹WAN Fang-zhu¹ TIAN Hua ^1*

1. Institute of Anatomy and Histology and Embryology, Shandong University School of Medicine, Ji’nan 250012, China;2. Department of Oncology, the First People’s Hospital of Tancheng, Shandong Linyi 276199, China;3. Department of Technical Support, Shandong University, Ji’nan 250012, China

Received:2013-11-01 Revised:2014-01-06 Online:2014-06-06 Published:2014-06-06
Contact: TIAN Hua E-mail:sduth@163.com

摘要/Abstract

摘要：

目的探讨基质细胞衍生因子-1α(SDF-1α)及白细胞介素-1β（IL-1β）诱导内皮细胞表达淋巴管表型的作用。方法 SDF-1α和IL-1β分别诱导内皮细胞株CRL-1730，用Real-time PCR、Western blotting及免疫细胞化学等方法检测其内皮及淋巴管标志物，的表达情况。结果 SDF-1α诱导培养之后，CRL-1730细胞株的内皮细胞标志物血管性血友病因子（vWF）、血管内皮钙黏蛋白（VE-cadherin）、血管内皮生长因子受体（VEGFR）2随其浓度增高而表达降低，淋巴管标志物平足蛋白（podoplanin）、同源异形盒蛋白-1（Prox-1）和淋巴管内皮透明质酸受体-1（LYVE-1）随其浓度增高而表达增高。IL-1β诱导之后，CRL-1730细胞株的vWF、VEGFR2和podoplanin、prox-1、LYVE-1的变化趋势同SDF-1α，而VE-cadherin的表达量基本不变。结论 SDF-1α和IL-1β都能够诱导血管内皮细胞表达淋巴管标志物。

关键词: 基质细胞衍生因子-1α, 白细胞介素-1β, , 同源异形盒蛋白-1, 血管内皮细胞, 实时定量聚合酶链反应

Abstract:

Objective To investigate the effect of stromal cell-derived factor-1α (SDF-1α) and interleukin(IL-1β) on inducing vascular endothelial cells to express lymphatic phenotype. Methods The CRL-1730 cell line was cultured and treated with SDF-1α or IL-1β. The expression of endothelial cell markers and lymphatic endothelial cell markers were investigated with Real-time PCR, Western blotting and immunocytochemistry. Results In CRL-1730 cell line, endothelial cell markers such as voln willebrand factor (vWF), VE-cadherin, vascular endothelial growth factor receptor(VEGFR)2, were dose dependently down-regulated after SDF-1α stimulation, while lymphatic phenotypes such as Prox-1, podoplanin and lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1), were dose-dependently up-regulated after SDF-1α stimulation. The changes of vWF, VEGFR2 and podoplanin, Prox-1, LYVE-1 expression after IL-1β stimulation was similar to that after SDF-1α while expression of VE-cadherin changed slightly. Conclusion SDF-1α and IL-1β are able to induce vascular endothelial cell expressing lymphatic phenotype.

Key words: Stromal cell-derived factor-1α, Interleukin-1β, Prospero ho-meobox protein-1, Vascular endothelial cell, Real-time PCR

索宁王雪颖杨春林周辉李菲张宗璞万芳竹田铧*. 基质细胞衍生因子-1α和白细胞介素-1β诱导淋巴管内皮表型的作用[J]. 解剖学报, 2014, 45(3): 388-392.

SUO Ning WANG Xue-ying YANG Chun-lin ZHOU Hui LI Fei ZHANG Zong-pu WAN Fang-zhu TIAN Hua*. Effect of stromal cell derived factor-1α and interleukin-1β on inducing vascular endothelial cells expressing lymphatic phenotype[J]. AAS, 2014, 45(3): 388-392.

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基质细胞衍生因子-1α和白细胞介素-1β诱导淋巴管内皮表型的作用

Effect of stromal cell derived factor-1α and interleukin-1β on inducing vascular endothelial cells expressing lymphatic phenotype

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