解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (3): 421-423.doi: 10.3969/j.issn.0529-1356.2014.03.023

• 技术方法 • 上一篇    下一篇

利用醛复红染色法制作家兔皮下疏松结缔组织铺片标本

杨世明*   

  1. 华中科技大学同济医学院解剖学系组织学胚胎学教研室，武汉 430030
  • 收稿日期:2013-12-24 修回日期:2014-02-21 出版日期:2014-06-06 发布日期:2014-06-06
  • 通讯作者: 杨世明 E-mail:ysm@mails.tjmu.edu.cn

Making stretched specimens by using aldehyde fuchsin staining on subcutaneous loose connective tissue in rabbits

YANG Shi-ming*   

  1. Division of Histology and Embryology, Department of Anatomy, Tongji Medical College，Huazhong University of Science and Technology,Wuhan 430030, China
  • Received:2013-12-24 Revised:2014-02-21 Online:2014-06-06 Published:2014-06-06
  • Contact: YANG Shi-ming E-mail:ysm@mails.tjmu.edu.cn

摘要：

目的 探讨适用于家兔皮下疏松结缔组织铺片标本的制片方法。方法 家兔3只，分别经腹腔注射10g/L锥虫蓝生理盐水溶液10～12ml。每天1次，连续注射3d后，于第4天取皮下疏松结缔组织进行铺片。待铺片晾干后，用福尔马林-酒精固定液固定约6h。然后，分别入醛复红、核固红和伊红染色液进行染色。每步之间用自来水冲洗。最后，常规脱水、透明、封片。 结果 巨噬细胞呈不规则形，分布在纤维中间，胞质中可见粗大的锥虫蓝颗粒。细胞核呈红色。醛复红染色30～40min时，弹性纤维呈紫色或蓝紫色，胶原纤维呈浅红色。结论 该法操作步骤简单，结果稳定可靠，是制作家兔皮下疏松结缔组织铺片标本适宜的方法。

关键词: 疏松结缔组织, 伊红, 弹性纤维, 胶原纤维, 巨噬细胞, 醛复红染色, 家兔

Abstract:

Objective To explore a simple and suitable method for stretch preparation of subcutaneous loose connective tissue in rabbits. Methods Three rabbits were continuously performed with 10-12ml of trypan blue saline solution(10g /L) by intraperitoneal injection once a day. The subcutaneous loose connective tissue was collected for stretch prepararation on the fourth day and fixed with a formalin-alcohol salution for 6 hours. The tissue was put into aldehyde fuchsin, nuclear fast red and eosin for staining.Washing with tap water was taken after every step. Finally, conventional dehydration, clearing and mounting were applied.
Results Macrophages were irregular in sharp and distributed among fibers. Many engulfed blue granules were observed within the cytoplasms. The nuclei were stained in red. The color of elastic fibers showed purple or blue-violet at the time point of aldehyde fuchsin staining between 30-40 minutes. The color of collagen fibers was light red. Conclusion The present method is simple, stable and reliable for stretch preparation of subcutaneous loose connective tissue in rabbits.

Key words: Loose connective tissue, Eosin, Elastic fiber, Collagen fiber, Macrophage, Aldehyde fuchsin staining, Rabbit