解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (4): 525-530.doi: 10.3969/j.issn.0529-1356.2014.04.015

• 肿瘤生物学 • 上一篇    下一篇

灵芝孢子油及灵芝提取物孢子油对乳腺癌血管生成调节因子的作用

张京1 牛苗苗1 杨丽1 范莉莎2 吴莉2 战军1* 张宏权1   

  1. 1. 北京大学基础医学院组织学与胚胎学教研室，北京 100191; 2. 深圳市康道生物有限公司，深圳 518001
  • 收稿日期:2014-02-21 修回日期:2014-06-23 出版日期:2014-08-06 发布日期:2014-08-06
  • 通讯作者: 战军 E-mail:zhanjun@bjmu.edu.cn
  • 基金资助:

    国家自然科学基金资助项目Kindlin-1和Kindlin-2调控肺癌细胞可塑性的分子机制及其作为肺癌分化和预后标志物的研究;教育部博士点基金;国家自然科学基金资助项目；重编程产生Claudin-low/三阴乳腺癌干细胞：乳腺癌可塑性的分子机制及转化医学意义;北京市与中央在京高校共建项目;北京大学中央高校基本科研业务费资助

Effects of ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil on angiogenesis regulatory factors

ZHANG Jing1 NIU Miao-miao1 YANG Li1 FAN Li-sha２ WU Li２ ZHAN Jun 1* ZHANG Hong-quan1   

  1. 1. Department of Histology and Embryology, School of Basic Medicine, Peking University, Beijing 100191, China;2. Shenzhen Kangdao Biological Co. Ltd. Shenzhen 518001, China
  • Received:2014-02-21 Revised:2014-06-23 Online:2014-08-06 Published:2014-08-06
  • Contact: ZHAN Jun E-mail:zhanjun@bjmu.edu.cn

摘要：

目的 探讨灵芝孢子油及灵芝提取物孢子油对人源高恶性乳腺癌血管生成调节因子的作用。方法 〖HTSS〗不同剂量的灵芝孢子油、灵芝提取物孢子油分别刺激体外培养的人源高恶性乳腺癌细胞系MDA-MB-231细胞及MDA-MB-231细胞制作的荷廇小鼠，Western blotting检测表皮生长因子受体（EGFR）的蛋白表达变化，Real-time PCR检测肿瘤血管生成相关因子EGFR、血管内皮生长因子（VEGF）、基质金属蛋白酶（MMPs）、血小板反应素-1（TSP-1）、血小板衍生因子（PDGF）、成纤维细胞生长因子（FGF）转录水平的基因表达变化。 结果 灵芝孢子油、灵芝提取物孢子油均可在体内和体外抑制人源高恶性乳腺癌细胞MDA-MB-231的EGFR蛋白表达，且有剂量依赖性；均可在体内和体外抑制VEGF RNA表达，增强血管生成抑制因子TSP-1 RNA表达；灵芝提取物孢子油比灵芝孢子油的作用更为显著。 结论 灵芝孢子油、灵芝提取物孢子油均有抑制肿瘤血管生成因子表达和促进血管生成抑制因子表达的作用, 其中灵芝提取物孢子油比灵芝孢子油的作用更为显著。

关键词: 灵芝孢子油, 灵芝提取物孢子油, 表皮生长因子受体, 血管内皮生长因子, 血小板反应素-1, 免疫印迹法

Abstract:

Objective To study the role of ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil in neovascularization of human high malignant breast cancer. Methods Human high malignant breast cancer cell MDA-MB-231 and tumor-bearing nude mice established with MDA-MB-231 were treated with different doses of ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil. Epidermal growth factor receptor(EGFR) expression level was examined by Western blotting and the RNA expression levels of neovascularization related molecules such as EGFR, vascular endothelial growth factor(VEGF), metalloproteinases(MMPs), thrombospondin(TSP-1), platelet derived growth factor(PDGF), fibroblast growth factor(FGF) were detected by Real-time PCR. Results Both ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil inhibited the expression of EGFR in vitroand in vivoin a dose-dependent way. Both compounds induced down-regulation of VEGF and up-regulation of TSP-1 at RNA level. The effect of Ganoderma lucidum extraction spore oil was more significant than that of ganoderma lucidum spore oil. Conclusion Both ganoderma lucidum spore oil and ganoderma lucidum extraction spore oil inhibite the expression of neovascularization related molecules and increase the expression of molecules inhibiting neovascularization, whereas the effect of ganoderma lucidum extraction spore oil is more obvious.

Key words: Ganoderma lucidum spore oil, Ganoderma lucidum extraction spore oil, Epidermal growth factor receptor, Vascular endothelial growth factor, Thrombospondin-1, Western blotting