解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (5): 591-598.doi: 10.3969/j.issn.0529-1356.2014.05.002

• 神经生物学 • 上一篇    下一篇

钠尿肽受体A在小鼠视网膜发育过程中的表达

李金菊 李瑞玲 李雪 刘恺 邓洁心 吴萍* 邓锦波*   

  1. 河南大学护理学院神经生物学研究所，河南 开封 475004
  • 收稿日期:2014-02-10 修回日期:2014-04-02 出版日期:2014-10-06 发布日期:2013-10-06
  • 通讯作者: 吴萍 邓锦波 E-mail:jinbo_deng@henu.edu.cn
  • 基金资助:

    Cajal-Retzius细胞促进移植干细胞在脑片上有序分化的实验研究;小脑平行纤维损伤及其再生后突触重建的活体纤维观察;胎儿酒精综合征对视皮质和海马锥体细胞树突棘和突触发育影响的研究

Expression of natriuretic peptide receptor A in the developing retina of the mouse

LI Jin-ju LI Rui-ling LI Xue LIU Kai Deng Jie-xin WU Ping* DENG Jin-bo*   

  1. Institute of Neurobiology, Nursing College, Henan University, He’nan Kaifeng 475004, China
  • Received:2014-02-10 Revised:2014-04-02 Online:2014-10-06 Published:2013-10-06
  • Contact: WU Ping DENG Jin-bo E-mail:jinbo_deng@henu.edu.cn

摘要：

目的 检测钠尿肽受体(NPR)在不同年龄小鼠视网膜内的表达，探讨其在视网膜发育过程中的作用。方法 收集从受孕16日(E16)到出生90日（P90）小鼠眼球标本共127只，对NPR-A进行免疫荧光检测。结果 NPR-A广泛存在于视网膜神经元中，例如，在外核层，NPR-A于P7开始高表达在视锥、视杆细胞内、外突起上，于P14减弱，P30之后持续稳定弱表达；在内核层，从P7开始NPR-A持续弱表达在双极细胞的突起中，而在水平细胞中未见NPR-A表达；在神经节细胞层，NPR-A于E16开始高表达在神经节细胞胞体中，P14明显减弱，而在神经纤维层，即神经节细胞的轴突中，NPR-A从胚胎期至成年持续高表达；在外网状层和内网状层，NPR-A于P14均高表达，但于P30之后逐渐减弱。此外，NPR-A还广泛的存在于Müller细胞的突起中。 结论 NPR-A参与了视网膜的发育，可能是小鼠视网膜神经元发育过程中的关键分子，并对Müller细胞的功能活动起着重要的调节作用。

关键词: 视网膜, 发育, 钠尿肽受体A, 免疫组织化学, 小鼠

Abstract:

Objective Our purpose is to investigate the expression of natriuretic peptide receptor A (NPR-A) in the retina and to understand the NPR-A’s functions during the mouse development. Methods Mice eyes were harvested from E16 (embryonic day 16) to P90 (postnatal day 90). Total of 127 eyes were used in the study. Immunohistochemistries of NPR-A were carried out. Results During development, NPR-A was widely expressed in the retinal neurons. In the outer nuclear layer, NPR-A began to appear in the inner and outer projections of cone and rod cells at P7, but decreased at P14. From P30 afterward, it continued to express weakly. In the inner nuclear layer, NPR-A expressed in the dendrites of bipolar cells weakly from P7 to adulthood, whereas no expression in horizontal cells. In the ganglion cell layer, NPR-A started highly to express in the ganglion cell bodies at E16, and in the meantime, in the nerve fiber layer, ganglion cell axons, NPR-A was expressed highly from embryonic to adult. In the inner and outer plexiform layers, NPR-A was highly expressed at P14, but decreased gradually after P30. In addition, NPR-A also widely expressed in the inner protrusions of Müller cells. Conclusion NPR-A participates in the development of the retina, and may be the key molecule in the developing retinal neurons. Moreover, it plays an important regulatory role in the functional activity of Müller cells.

Key words: Retina, Development, Natriuretic peptide receptor A, Immunohistochemistry, Mouse