解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (6): 793-799.doi: 10.3969/j.issn.0529-1356.2014.06.011

• 细胞和分子生物学 • 上一篇    下一篇

姜黄素对猪骨髓间充质干细胞增殖和成脂分化的影响

张庆美 李方正 姜忠玲 孙东兴 李东建 宋学雄*   

  1. 青岛农业大学动物科技学院，山东 青岛 266109
  • 收稿日期:2014-04-16 修回日期:2014-05-21 出版日期:2012-12-06 发布日期:2014-12-06
  • 通讯作者: 宋学雄 E-mail:935659360@qq.com
  • 基金资助:

    国家自然基金

Effects of curcumin on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stem cells

ZHANG Qing-mei LI Fang-zheng JIANG Zhong-ling SUN Dong-xing LI Dong-jian SONG Xue-xiong*   

  1. College of Animal Science and Technology, Qingdao Agricultural University, Shandong Qingdao 266109, China
  • Received:2014-04-16 Revised:2014-05-21 Online:2012-12-06 Published:2014-12-06
  • Contact: SONG Xue-xiong E-mail:935659360@qq.com

摘要：

目的 探讨姜黄素(curcumin)对猪骨髓间充质干细胞(BMSCs)增殖和成脂分化的影响及机制。方法 用含不同浓度姜黄素的细胞培养液和诱导分化液，培养和诱导分化猪BMSCs，用MTT比色法检测对BMSCs增殖的影响，用形态学和油红O染色提取法检测对成脂分化的影响，用实时荧光定量PCR（Real-time PCR）检测对成脂分化相关基因过氧化物酶体增殖物激活受体γ（PPARγ2）和脂蛋白脂肪酶（LPL） mRNA表达的影响。 结果 当姜黄素用于猪BMSCs的培养，1μmol/L浓度在培养第3天、第5天和10μmol/L浓度在培养第5天开始能够显著促进细胞增殖（P＜0.05），但高于15μmol/L则具有显著的抑制作用（P＜0.05）；当姜黄素用于猪BMSCs的成脂诱导分化，所用不同浓度的姜黄素都能显著抑制成脂分化（P＜0.05），用0.5μmol/L获得28.3%的抑制率，而用20μmol/L获得71.24%的高抑制率；当用20μmol/L姜黄素处理和分别诱导分化5、10和15d，PPARγ2 mRNA表达的抑制率分别达到19.11%、49.52%和76.76%，LPL mRNA表达的抑制率分别达到37.58%、49.32%和74.70%。 结论 适当浓度姜黄素具有促进猪BMSCs增殖和抑制猪BMSCs向脂肪细胞分化的作用，对脂肪细胞的分化发挥了重要调控作用。

关键词: 骨髓间充质干细胞, 姜黄素, 增殖, 成脂分化, 实时定量聚合酶链反应, 猪

Abstract:

Objective To investigate the effects and mechanisms of curcumin on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stem cells (BMSCs). Methods The porcine BMSCs were cultured and differentiated with cell culture and adipegenic differentiation medium containing different concentrations of curcumin, the effects of curcumin on proliferation of porcine BMSCs were detected by MTT, the effects of curcumin on adipogenic differentiation were analyzed by morphology and oil red O, and the expressions of peroxisome proliferator-activated receptor γ(PPARγ2) mRNA and lipoprotein lipase(LPL) mRNA were detected by Real-time PCR. Results When the porcine BMSCs were cultured with curcumin, 1μmol/L curcumin at the third days of culture and 5, 10μmol/L curcumin at the fifth days of culture significantly promoted cell proliferation (P<0.05), but curcumin concentration higher than 15μmol/L inhibited cell proliferation (P<0.05). When the porcine BMSCs were differentiated with curcumin, the different concentrations of curcumin significantly inhibited adipogenic differentiation (P<0.05). The inhibition rate of 0.5μmol/L curcumin was 28.3% and 20μmol/L curcumin was 71.24%. When the cells were treated with 20μmol/L curcumin and differentiated in 5, 10 and 15 days, the inhibition rates of expressions of PPARγ2 and LPL mRNA were 19.11%, 49.52%, 76.76% and 37.58%, 49.32%, 74.70% respectively.
Conclusion The proper concentrations of curcumin were able to promote the proliferation and inhibit adipogenic differentiation of porcine BMSCs, suggesting that curcumin may play an important role in the regulation of adipocyte differentiation.

Key words: Bone mesenchymal stem cell, Curcumin, Proliferation, Adipogenic differentiation, Real-time PCR, Porcine