佛手苷内酯通过调控长链非编码RNA 阿片样受体基因表达对糖氧剥夺诱导的PC12细胞损伤的影响

doi:10.16098/j.issn.0529-1356.2023.01.008

摘要/Abstract

摘要：

目的研究佛手苷内酯（BG）是否通过调控长链非编码RNA（lncRNA）阿片样受体基因（Oprm1）表达保护糖氧剥夺（OGD）诱导的PC12细胞损伤。方法将PC12细胞分为对照组（Con）、OGD组、OGD+ BG低浓度（BG-L）组、OGD+ BG中浓度（BG-M）组、OGD+ BG高浓度（BG-H）组、OGD+pcDNA组、OGD+pcDNA-Oprm1组、OGD+BG+si-NC组和OGD+BG+si-Oprm1组。试剂盒测定丙二醛（MDA）含量、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性。流式细胞术分析细胞凋亡率。Real-time PCR分析Oprm1表达水平。结果与Con组比较，OGD组PC12细胞凋亡率和MDA含量显著升高，Oprm1表达、SOD和GSH-Px活性显著降低（P<0.05）。与OGD组比较，OGD+BG-L组、OGD+BG-M组、OGD+BG-H组PC12细胞凋亡率和MDA含量显著降低，Oprm1表达、SOD和GSH-Px活性显著升高（P<0.05）。与OGD+pcDNA组比较，OGD+pcDNA-Oprm1组PC12细胞凋亡率和MDA含量显著降低，SOD和GSH-Px活性显著升高（P<0.05）。与OGD+BG+si-NC组比较，OGD+BG+si-Oprm1组PC12细胞凋亡率和MDA含量显著升高，SOD和GSH-Px活性显著降低（P<0.05）。结论 BG可减轻OGD诱导的PC12细胞损伤，其机制与上调lncRNA Oprm1表达有关。

关键词: 佛手苷内酯, 糖氧剥夺损伤, 长链非编码RNA, 阿片样受体基因, 流式细胞术, 实时定量聚合酶链反应, PC12细胞

Abstract:

Objective To study whether bergapten (BG) protects PC12 cells from oxygen-glucose deprivation (OGD) induced cell injury by regulating long non-coding RNA (lncRNA) opioid receptor gene (Oprm1) expression. Methods PC12 cells were divided into control (Con) group, OGD group, OGD+ low concentration BG (BG-L) group, OGD+medium concentration BG (BG-M) group, OGD+high concentration BG (BG-H) group, OGD+ pcDNA group, OGD+pcDNA-Oprm1 group, OGD+BG+si-NC group, OGD+BG+si-Oprm1 group. The malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were measured by the kits. Cell apoptosis rate was analysed by flow cytometry. The expression level of Oprm1 was analysed by Real-time PCR. Results Compared with the Con group, the apoptosis rate and MDA content of PC12 cells in OGD group increased significantly, whereas Oprm1 expression, SOD and GSH-Px activity decreased significantly (P<0.05). Compared with the OGD group, the apoptosis rate and MDA content of PC12 cells in the OGD+BG-L group, OGD+BG-M group, OGD+BG-H group were significantly reduced, whereas the Oprm1 expression, SOD and GSH-Px activities increased significantly (P<0.05). Compared with the OGD+pcDNA group, the apoptosis rate and MDA content of the PC12 cells in the OGD+pcDNA-Oprm1 group reduced significantly, whereas the SOD and GSH-Px activities increased significantly (P<0.05). Compared with the OGD+BG+si-NC group, the apoptosis rate and MDA content of PC12 cells in the OGD+BG+si-Oprm1 group increased significantly, whereas the SOD and GSH-Px activities decreased significantly (P<0.05). Conclusion Bergapten may alleviate OGD-induced PC12 cell injury, which is correlated to the up-regulation of lncRNA Oprm1 expression.

Key words:

Bergapten, Glucose and oxygen deprivation injury, Long non-coding RNA, Opioid receptor gene, Flow cytometry, Real-time PCR, PC12 cell

中图分类号:

R743

孙孟坊程一升王丰金孟浩汪德秀. 佛手苷内酯通过调控长链非编码RNA 阿片样受体基因表达对糖氧剥夺诱导的PC12细胞损伤的影响[J]. 解剖学报, 2023, 54(1): 56-62.

SUN Meng-fang CHENG Yi-sheng WANG Feng JIN Meng-hao WANG De-xiu.

Effect of bergapten on glucose and oxygen deprivation induced PC12 cell injury by regulating long non-coding RNA opioid receptor gene expression

[J]. Acta Anatomica Sinica, 2023, 54(1): 56-62.

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