天麻素抑制脂多糖诱导的人脐静脉内皮细胞的自噬

doi:10.3969/j.issn.0529-1356.2014.06.016

解剖学报 ›› 2014, Vol. 45 ›› Issue (6): 824-829.doi: 10.3969/j.issn.0529-1356.2014.06.016

• 组织学胚胎学发育生物学 • 上一篇下一篇

天麻素抑制脂多糖诱导的人脐静脉内皮细胞的自噬

解亚男¹ 杨萍¹文飞¹孙林²陈媛丽^1* 陆地^1*

1.昆明医科大学人体解剖学与组织学胚胎学系，昆明 650500; 2. 昆明医科大学第二附属医院心内科，昆明 650101

收稿日期:2014-03-31 修回日期:2014-06-20 出版日期:2012-12-06 发布日期:2014-12-06
通讯作者: 陈媛，陆地 E-mail:ludi20040609@126.com
基金资助:
昆明医科大学研究生创新基金项目;国家自然科学基金资助项目;云南省应用基础研究重点项目

Gastrodin inhibits lipopolysaccharide-induced autophagy in human umbilical vein endothelial cells

XIE Ya-nan¹ YANG Ping¹ WEN Fei¹ SUN Lin² CHEN Yuan-li ^1* LU Di ^1*

1. Department of Human Anatomy and Histological Embryology，Kunming Medical University， Kunming 650500，China;2. Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China

Received:2014-03-31 Revised:2014-06-20 Online:2012-12-06 Published:2014-12-06
Contact: CHEN Yuan-li ， LU Di E-mail:ludi20040609@126.com

摘要/Abstract

摘要：

目的探讨天麻素(Gas)在脂多糖 (LPS) 诱导的人脐静脉内皮细胞 ( HUVECs) 自噬中的作用。方法用不同浓度的Gas(5、10、20、50μmol/L) 预处理 HUVECs 1h，再加入1mg/L LPS共培养 12 h后,分别提取细胞总蛋白及mRNA，采用 Western blotting 及 RT-PCR 检测 Beclin-1、ATG5 及 LC3的表达变化；采用丹酰尸胺( MDC) 染色检测细胞中自噬小体(autophagosome)的表达变化；采用MTT检测 HUVECs 生存率的变化。结果 LPS 诱导 HUVECs 产生自噬；Gas 预处理后自噬水平明显降低且呈剂量依赖性；与此同时细胞的生存率明显增加。结论 LPS诱导 HUVECs 产生自噬；Gas 通过抑制由LPS 诱导产生的自噬，提高 HUVECs 的生存率。

关键词: 天麻素, 脂多糖, 自噬, 丹酰尸胺, 免疫印迹法, 反转录-聚合酶链反应, 人脐静脉内皮细胞

Abstract:

Objective To investigate the regulation mechanism of gastrodin (Gas) on atutophagy of human umbilical vein endothelial cells（HUVECs）stimulated with lipopolysaccharide (LPS). Methods Gas at 5, 10, 20 and 50μmol/L was added 1h before 1mg/L LPS stimulation, after 12h incubation. The protein and mRNA expression of Beclin-1, ATG5 and LC3 were detected by Western blottingting and RT-PCR respectively. The autophagosome was observed under fluorescent microscope by monodansylcadaverine (MDC) staining. Cell viability rate was measured by MTT analysis. Results Autophagy was induced in HUVECs by LPS-stimulation. The protein and mRNA expression of Beclin-1, ATG5 and LC3 were significantly increased,which was in a time-dependent manner. Compared with LPS-stimulated group, pertreatment with Gas inhibited the expression of autophagy in dose-dependent manner and the cell viability was effectively increased. Conclusion Autophagy was induced in HUVECs by LPS-stimulation. Gas may increase the viability rate of HUVECs through inhibiting the expression of autophagy.

Key words: Gastrodin, Lipopolysaccharide, Autophagy, Monodansylcadaverine, Western blotting, RT-PCR, Human umbilical vein endothelial cell

解亚男杨萍文飞孙林陈媛丽* 陆地*. 天麻素抑制脂多糖诱导的人脐静脉内皮细胞的自噬[J]. 解剖学报, 2014, 45(6): 824-829.

XIE Ya-nan YANG Ping WEN Fei SUN Lin CHEN Yuan-li* LU Di*. Gastrodin inhibits lipopolysaccharide-induced autophagy in human umbilical vein endothelial cells[J]. AAS, 2014, 45(6): 824-829.

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天麻素抑制脂多糖诱导的人脐静脉内皮细胞的自噬

Gastrodin inhibits lipopolysaccharide-induced autophagy in human umbilical vein endothelial cells

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