解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (6): 830-834.doi: 10.3969/j.issn.0529-1356.2014.06.017

• 组织学胚胎学发育生物学 • 上一篇    下一篇

基质金属蛋白酶-9和血管内皮生长因子在子痫前期患者胎盘组织中的表达

花茂方1* 刘小云2 李善凤1   

  1. 1. 连云港市妇幼保健院妇科， 江苏  连云港 222006; 2. 徐州医学院附属医院中心实验室， 江苏 徐州 221000
  • 收稿日期:2014-04-17 修回日期:2014-07-22 出版日期:2012-12-06 发布日期:2014-12-06
  • 通讯作者: 花茂方 E-mail:18705130566@163.com
  • 基金资助:

    江苏省连云港市科技局

Expressions of the RECK, matrix metalloproteinases-9 and vascular endothelial/growth factor in placentas of preeclamptic patients

HUA Mao-fang 1* LIU Xiao-yun2 LI SHan-feng1   

  1. 1. Department of Gynaecology, Lianyungang Maternal and Child health Hospital, Jiangsu Lianyungang 222006, China; 2. Centre Laboratory,  Affiliated Hospital of Xuzhou Medical College, Jiangsu Xuzhou 221000, China
  • Received:2014-04-17 Revised:2014-07-22 Online:2012-12-06 Published:2014-12-06
  • Contact: HUA Mao-fang E-mail:18705130566@163.com

摘要：

 目的 检测子痫前期患者胎盘组织中RECK、基质金属蛋白酶-9（MMP-9）、血管内皮生长因子（VEGF）的基因表达，探讨它们与胎盘滋养细胞浸润过程的调控关系。 方法 采用RT-PCR、Western blotting、免疫组织化学检测120例妊娠足月剖宫产（正常妊娠、轻度子痫前期、中度子痫前期、重度子痫前期各30例）胎盘组织中RECK、MMP-9、VEGF的基因表达。结果 3组子痫前期患者胎盘组织中MMP-9及VEGF的mRNA表达水平均显著低于正常妊娠组（P<0.05）；重度子痫前期组中RECK mRNA的表达显著高于正常妊娠组（P<0.05）；3组子痫前期患者胎盘组织中MMP-9及VEGF蛋白表达均显著低于正常妊娠组（P<0.05），中度和重度子痫前期组中RECK蛋白表达显著高于正常妊娠组（P<0.05）。结论 子痫前期患者胎盘中RECK与MMP-9、VEGF之间存在负相关性，它们可能参与了胎盘滋养细胞浅浸润过程的调控。

关键词: RECK基因, 基质金属蛋白酶9, 血管内皮生长因子, 子痫前期, 胎盘, 免疫印迹法, 免疫组织化学, 人

Abstract:

Objective To investigate the expression of reversion-inducing cysteine-rich protein with reversion-inducing-cysteine-rich protein with kazal motifs (RECK), matrix metalloproteinase-9 (MMP-9) as well as vascular endothelial growth factor (VEGF) genes in placenta of preeclamptic patients, and discuss the regulation of these genes in invasion of placenta trophoblasts. Methods Reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry were employed in the detection of expression of RECK gene, MMP-9 and VEGF in placenta tissues from 120 gravidae with full-term pregnancy undergoing cesarean section, i.e., normal pregnancy group (n=30), mild preeclampsia group (n=30), moderate preeclampsia group (n=30) and severe preeclampsia group (n=30). Results The mRNA expression level of MMP-9 and VEGF in three preeclampsia groups were all significantly lower (P<0.05) than those in normal pregnancy group. The expression level of RECK mRNA in the severe preeclampsia group was significantly higher (P<0.05) than that in normal pregnancy group. The expression of MMP-9 and VEGF proteins in three preeclampsia groups were lower than those in normal pregnancy group with statistically significant differences (P<0.05)，and the expression of RECK protein in moderate and severe preeclampsia groups were both significantly higher (P<0.05) than that in normal pregnancy group. Conclusion The expression level of RECK is negatively correlated with those of MMP-2 and MMP-9 in preeclamptic patients, which indicates that they are involved in regulating the process of shallow invasion of trophoblasts in placenta.

Key words: RECK gene, Matrix metalloproteinase-9, Vascular endothelial growth factor, Preeclampsia, Placenta, Western blotting, Immunohistochemistry, Human