›› 2007, Vol. 38 ›› Issue (1): 34-37.doi:
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崔景彬1,2;张亚卓1*;孙梅珍1;何乐1;李丹1
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摘要: P>
关键词: P>胰岛素样生长因子1, β-淀粉样蛋白, 神经干细胞, 凋亡, 免疫细胞化学, 大鼠/P>
Abstract: P>Objective To observe the influences of βamyloid peptide(Aβ) on the proliferation and apoptosis of neural stem cells(NSCs) and to investigate the protective mechanism of insulinlike growth factor1(IGF-1) from toxicity of Aβ. Methods NSCs were isolated from E14 SD rats brains and then treated with IGF-1, Aβ and IGF-1 plus Aβ respectively. The cells were dyed with trypanblue for the count of dead cells and the death rate. BrdU was used to label cells and to evaluate cell proliferation. NSCs and newborn cells were identified by immunocytochemistry and apoptosis was detected by TUNEL technique. Results Cell death rate was low and there were many BrdUpositive cells, but no TUNELpositive cells in the medium containing IGF-1. Cell death rate increased strikingly 6-48 hours after Aβ was added, and a great many TUNELpositive cells were observed in the group treated with Aβ. Death rate of cells and TUNELpositive cell
Key words: P>Insulinlike growth factor1, βamyloid peptide, Neural stem cell, Apoptosis, Immunocytochemistry, Rat/P>
中图分类号:
R338.2、R745.7
崔景彬;张亚卓;孙梅珍;何乐;李丹. P>胰岛素样生长因子-1抑制β淀粉样蛋白25~35诱导的神经干细胞凋亡/P>[J]. , 2007, 38(1): 34-37.
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https://jpxb.bjmu.edu.cn/CN/Y2007/V38/I1/34