解剖学报

›› 2007, Vol. 38 ›› Issue (2): 243-245.doi:

• 论著 • 上一篇    下一篇

大鼠发情周期下丘脑中食欲素及其受体的变化

王俊波 1 ;王培军 2; 凌树才 1*; 沈岚 3; 孙百强 1 ;朱晞1   

  1. 1 浙江大学医学院解剖学教研室，杭州 310031； 2 佳木斯大学基础医学院解剖学教研室，佳木斯 154007；3 杭州市红十字会医院妇产科，杭州 310004
  • 收稿日期:2006-07-21 修回日期:2006-12-08 出版日期:2007-04-06
  • 通讯作者: 凌树才

CHANGES OF OREXIN AND ITS RECEPTORS IN THE RAT HYPOTHALAMUS DURING ESTROUS CYCLE

  1. 1Department of Anatomy, Medical College of Zhejiang University, Hangzhou 310031, China;2Department of Anatomy, Medical College of Jiamusi University, Jiamusi 154007, China;3Red Cross Hospital of Hangzhou, Hangzhou 310004, China
  • Received:2006-07-21 Revised:2006-12-08 Online:2007-04-06
  • Contact: LING Shu-cai

关键词: 食欲素, 食欲素受体, 下丘脑, 发情周期, 竞争性逆转录多聚酶链式反应, 大鼠

Abstract: Objective To investigate a possible role of orexin in the regulation of estrous cycle by examining the expression of orexin and orexin receptors(OX1R,OX2R) in the rat hypothalamus during the estrous cycle. Methods The levels of prepro-orexin(prepro-OX),orexin-A,OX1R,and OX2R in the rat hypothalamus during the estrous cycle were evaluated by competitive reverse transcription-polymerase china reaction (RT-PCR) assay. Results Only expression OX1R mRNA during late proestrus was significantly higher than that at metestrus.No significant changes of prepro-OX and OX2R mRNA expression were observed during the various estrous cycle phases.Conclusion Orexin might regulate the secretion of GnRH and/or LH by binding OX1R, contributing to the occurrence of ovulation.

Key words: Orexin(hypocretin), Orexin receptor, Hypothalamus, Estrous cycle, Competitive RT-PCR, Rat

中图分类号: