关键词: 坐骨神经切断, Sre抑制的蛋白激酶C的底物, 免疫组织化学, 免疫印迹法, 大鼠

Abstract: Objective To investigate the change of SSeCKS in sciatic nerve after the transection of rat sciatic nerve and its significance. Methods The sciatic nerve transection model of Sprague_Dawley rat was made. We tested the change of SSeCKS by Western blotting and immunohistochemistry. Results It showed from Western blotting that after injury, SSeCKS in the proximal lesion site increased gradually and reached its peak at 2d, then it began to decrease,while in the distal lesion site, SSeCKS peaked at 12h and then declined. Immunohistochemistry showed SSeCKS mainly located in break site, in a cluster expression pattern, while in the uninjury region, the level of SSeCKS was significantly lower and the distribution was uniform. The result of double immunofluorescent staining showed that SSeCKS partly colocalized with S100, NF200 and GAP43.Conclusion The transection of rat sciatic nerve can cause the change of SSeCKS and it might be involved in transduction of some harm stimulate signal moleculars after the injury. It may be also related with the nerve regeneration and function repair.

Key words: Sciatic nerve transection, SSeCKS, Immunohistochemistry, Western blotting, Rat