关键词: 复方鳖甲软肝方, 肝星形细胞, 转化生长因子-β1, Smad-3蛋白, 免疫组织化学, 大鼠

Abstract: Objective To investigate the effect of fufangbiejiaruanganfang drug serum on the expression of TGF-β type I receptor and Smad-3 protein in rat hepatic stellate cells(HSCs). Methods Immunocytochemical staining and image analysis were applied to investigate the effect of different dosages of fufangbiejiaruanganfang drug serums on the expression of TGF-β type I receptor and Smad-3 protein in rat HSCs. Results High and medium dosage of fufangbiejiaruanganfang drug serum remarkably downregulated the expression of TGF-β type I receptor and Smad-3 protein in rat HSCs in a dosedependent manner. Conclusion The experiment demonstrated that fufangbiejiaruanganfang drug serums can downregulate the expression of TGF-β type I receptor and Smad-3 protein in HSCs and further inhibit the activation of HSCs and improve liver fibrosis. So the downregulation of TGF-β type I receptor in HSCs may be one of

Key words: Fufangbiejiaruanganfang, Hepatic stellate cell (HSCs), Transforming growth factorβ1(TGF-β1), Smad-3 protein, Immunohisochemistry, Rat