关键词: 干细胞, 诱导分化, 多巴胺能神经元, 羊膜上皮细胞, 免疫组织化学, 人脐带血

Abstract: Objective To investigate the function of amnion endothelial cell in the differentiation of human umbilical cord blood stem cells into dopaminergic neurons. Methods Primary human amnion endothelial cells were separated and cultured in vitro; the conditioned medium(CM) was prepared through high speed centrifugation. The cord blood mesenchymal stem cells of PSUB>1/SUB> passage were induced by the conditioned medium, and the mophology of cells was observed under the inverted phase contrast microscope. The expression of tyrosine hydroxylase（TH）and dopamine transportor (DAT) of the induced cord blood mesenchymal stem cells were detected by immunocytochemistry staining method and immunoblotting(Western blotting). Results The masculine rate of TH and DAT of the cord blood mesenchymal stem cells of PSUB>1/SUB> passage which were induced by amnion endothelial cells conditioned medium was higher than that of the control group, with a significant difference (EM>P/EM>0.01 ). The results of Western blotting were in agreemetn with the immunocytochemistry staining. Conclusion Amnion endothelial cells can induce human umbilical cord blood stem cel

Key words: Stem cells, Inducing Differentiation, Dopaminergic neurons, Amnion endothelial cells, Immunohistochemistry, Human umbilical cord blood