关键词: 胰岛, 胰腺干细胞, 糖尿病, 免疫组织化学, 胎猪

Abstract: Objective To find a method for proliferating porcine fetus islet stem cells. Methods Cells collected from porcine fetus pancreas were digested with trypsine and cultured in medium RPMI1640 with 10% FBS. Some cells survived after seven days, these cells could be passed for 5 to 6 times. Then, most of those cells were trend to die and float in the medium, only were a few little cells attached on culture plate. These little cells proliferated quickly when medium was changed to RPMI 1640 with 15% FBS and each of the little cells can formed cells’ clone in 4 days. The cell’s character of islet stem cell was detected by immunochemical method. Results Cells obtained by the method posses strong proliferation ability and it has been sub cultured over 64 passages till now. It expressed islet stem cell’s mark such as PDX-1，GLUT-2，nestin，vimentin et.al. After induction, it expressed insulin, glucagon, somatostatin and polypeptide. Conclusion Cells obtained by this method were determined as islet stem cells. They can differentiate into islet endocrine cell EM>in vitro/EM>. This study can provide reference for isolation and expansion of isl

Key words: Islet, Islet stem cell, Diabetes, Immunohistochemistry, Porcine fetus