关键词: 细胞基质, Sylgard184, 相容性, 流术细胞术, C2C12细胞

Abstract: Objective An attempt was undertaken to acquire the ideal matrix material that can enhance the compatibility between silicone rubber elastomer(sylgard184) and murine C2C12 cells. Methods Two-component of Sylgard184 admixture to the ratio of 10∶1 was made then mixed into 4 wells of 6 Orifice plastic plate, solidification was carried out at room temperature, and the remaining 2 holes without coating were taken as blank control (group A).The surfaces of 4 wells were coated by collagen type I, laminin and poly-lisine, respectively as group B, C and D,and the wells of uncoating as group E,every group had six samples. C2C12 cells were cultured on the surface of Sylgard184 coated with different matrix material, Cell proliferation, differentiation morphology of five groups of C2C12 cells were observed by the inverted microscopy. Flow cytometry (FCM) was used to detect the cell generation cycle of C2C12 cells after enrichment culture 48 hours.RT-PCR was used to test MyoD, myogenin mRNA expression, cell proliferation or differentiation cultured for 48 hours respectively that growed on coated or uncoated surfaces of Sylgard184.Results Sylgard184 showed the cytotoxicity, E group C2C12 cells were all floating and dead 24 hours after seeded.D group cells were only a few survived. While B and C groups can significantly reduce the toxicity of Sylagard184, and strengthen the compatibility between their surface and C2C12 cells. The ratio of S-phase cell in group C coated with laminin was significantly higher than that of in group A and B (P<0.05),as well as the mRNA expression of MyoD and Myogenin. Conclusion The data suggest that Sylgard184 modified by laminin is superior to that modified by other biomaterials, in terms of which can enhance the proliferation and differentiation of cultured C2C12 cells EM>in vitro./EM>

Key words: Extracellular matrix, Sylgard184, Compatibility, Flow cytometry, C2C12 cell