解剖学报

›› 2009, Vol. 40 ›› Issue (2): 307-311.doi: 10.3969/j.issn.0529-1356.2009.02.028

• 论著 • 上一篇    下一篇

人胎儿胸腺内CD80和癌胚抗原的表达及定位

曹阳1;宫璀璀1*; 吴景兰2; 王一菱2 ;郑乃刚3 ;高峰4   

  1. 1.解放军第153中心医院检验科，郑州 450042;2.郑州大学组织学胚胎学教研室，郑州 450052;3.郑州大学肿瘤基础学研究室，郑州 450001; 4.河南省郑州大学医药科学研究院, 郑州 450052
  • 收稿日期:2007-11-26 修回日期:2007-12-10 出版日期:2009-04-06
  • 通讯作者: 宫璀璀

Expression and localization of CD80 and CEA in human fetal thymus

  1. 1. Laborary Center of the 153rd PLA Hospital, Zhengzhou 450042, China; 2. Department of Histology and Embryology, Zhengzhou University, Zhengzhou 450052, China; 3.Department of Basic Science of Oncology, Zhengzhou University, Zhengzhou 450001, China; 4.He′nan Institute of Medical Science, Zhengzhou University, Zhengzhou 450052, China
  • Received:2007-11-26 Revised:2007-12-10 Online:2009-04-06
  • Contact: GONG Cui-cui

关键词: 胸腺, CD80, 癌胚抗原, α-醋酸萘酯酶, 免疫组织化学, 免疫印迹法, 人胎儿

Abstract: Objective To explore the relationship between the expression level and localization of CD80 and carcinoembryonic antigen(CEA) in the human fetal thymus and its development. Methods Twelve aborted dead fetus samples(via donation from the dead fetus’s parents and permission of the hospital agency) were collected from Department of Genecology and Obstetrics in the affiliated hospital, including 4 cases aged less than 4 months in earlier stage and 8 cases aged 5 months in later stage. The two groups of earlier and later aged samples were randomly divided into two aliquots. One aliquot was prepared for frozen sections stained with HE, ANAE histochemistry, CD80 and carcinoembryonic antigen (CEA) immunohistochemistry; and detected with CEA ELISA. The protein extracted from another aliquot with Trizol reagent was detected with CD80 immunoblotting and CEA ELISA. Results The thymus in earlier aged group, especially the medulla was less developed. The reticulo-epithelial cells (RECs) with CD80IR in the later aged group could be classified into six patterns. The type I RE in flatten shape were located at the inner surface of the capscule and trabecula. The type II RECs (DCs) in small satellite shaped were localized in the medulla. Both type III and type IV RECs were located at the dzemarcation between the cortex and medulla. The type V RECs (DCs) in large satellite shaped with delicate processes to connect into reticular meshes were distributed in the medulla and the type VI RECs were found in the Hassall’s corpuscles. The ANAEdot pattern T cells (CD4SUP>+ /SUP>) and ANAE-diffuse granular pattern T cells (CD8SUP>+/SUP> ) in separate clusters could be found in the reticular pores. In the immunoblotting and ELISA the expression level of both CD80-IR and CEA-IR in the later aged group was higher than that in the earlier aged group.Conclusion The results suggest that the expression and cocalization of CD80 and CE

Key words: Thymus, CD80, CEA, ANAE, Immunohistochemistry, Western blotting, Human fetus

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