解剖学报

›› 2009, Vol. 40 ›› Issue (4): 680-684.doi: 10.3969/j.issn.0529-1356.2009.04.032

• 技术方法 • 上一篇    下一篇

羧基荧光素乙酰乙酸与四甲基偶氮唑盐法检测人脐血间充质干细胞刺激脾淋巴细胞增殖作用的比较

孙海梅1; 季凤清1* ;王屹3 ;王丹妮1; 赵春礼2 ;杨慧2   

  1. 1. 首都医科大学组织学与胚胎学教研室;2. 首都医科大学北京神经科学研究所， 北京 100069;3. 中国康复研究中心检验科，北京 100068
  • 收稿日期:2008-04-23 修回日期:2008-06-18 出版日期:2009-08-06
  • 通讯作者: 季凤清

Compararative investigation of proliferation of lymphocytes stimulated by human umbilical cord blood mesenchymal stem cells with CFSE and MTT

  1. 1. Department of Histology and Embryology, Capital Medical University, Beijing 100069, China; BR>2. Beijing Institute for Neuroscience Capital Medical Unviersity, Beijing 100069, China； 3. China Rehabilitation Reserch Center Medical Laboratory, Beijing 100068, China BR>
  • Received:2008-04-23 Revised:2008-06-18 Online:2009-08-06
  • Contact: JI Feng-qing1

关键词: 脐血间充质干细胞, 淋巴细胞, 增殖, 羧基荧光素乙酰乙酸, 四甲基偶唑盐比色法

Abstract: Objective To compare the sensitivities of the stimulation effect of human cord blood mesenchymal stem cells(CB-MSCs) and CB-MSCs of neuronal differentiation to lymphocytes(LCs) detected with carboxyfluorescein didcetate(CFSE) and MTT. Methods To prepare LCs from SD rat and divided into four group stimulating cells: 1. CB-MSCs; 2. Dif-CB-HSCs; 3. SH-SY5Y（positive control）; 4. Auto-LC（negative control）.Stimulating cells were respectively Co-cultured with LCs. The proliferation of LCs was detected with MTT and CFSE (EM>n/EM>=3). Results CB-MSCs and Dif-CB-HSCs stimulated LCs to proliferate more weakly than positive control detected with MTT and CFSE. The quantity of proliferation of lymphocytes Co-cultured with CB-MSCs and Dif-CB-HSCs were higher than that of Auto-LC detected with CFSE. But MTT OD value of CB-MSCs and Dif-CB-HSCs was a little lower than that of Auto-LC. Statistical analysis results showed no significant difference.Conclusion CFSE can reflect proliferation status of lymphocytes better than MTT. CFSE shows mor

Key words: Umbilical cord blood mesenchymal stem cell, Lymphocyte, Proliferation, Carboxyfluorescein didcetate, MTT assay

中图分类号: