解剖学报

解剖学报 ›› 2022, Vol. 53 ›› Issue (5): 607-612.doi: 10.16098/j.issn.0529-1356.2022.05.010

• • 上一篇    下一篇

微小RNA-29a-3p靶向Serpinh1调控人胃癌细胞系BGC823增殖和侵袭的分子机制

严晓娣1 薛强1 王斐然1 顾红梅1 唐翀2 刘睿祺3 周友浪1 刘贤称1*
  

  1. 1.南通大学附属医院肿瘤中心，江苏 南通 226000 ; 2.南通市第一人民医院胃肠外科，江苏 南通 226000; 3.南通大学医学院2021级，江苏 南通 226000
  • 收稿日期:2021-09-22 修回日期:2022-01-04 出版日期:2022-10-06 发布日期:2022-10-06
  • 通讯作者: 刘贤称 E-mail:liuxc@ntu.edu.cn
  • 基金资助:
    南通市市级科技计划项目;江苏省“六大人才高峰”项目

Molecular mechanism of microRNA-29a-3p targeting Serpinh1 regulating proliferation and invasion of human gastric cancer cell line BGC823

YAN  Xiao-di1  XUE Qiang1  WANG Fei-ran1  GU  Hong-mei1  TANG Chong2  LIU  Rui-qi3  ZHOU  You-lang1  LIU  Xian-cheng1*   

  1. 1.Department of Cancer Center, Affiliated Hospital of Nantong University, Jiangsu Nantong 226000, China ; 2.Department of Gastrointestinal Surgery, Nantong First People’s Hospital, Jiangsu Nantong 226000, China; 3.Grade 2021, Nantong Univeristy Medical School, Jiangsu Nantong 226000, China
  • Received:2021-09-22 Revised:2022-01-04 Online:2022-10-06 Published:2022-10-06
  • Contact: LIU Xian-cheng E-mail:liuxc@ntu.edu.cn

PDF

摘要：

目的  探讨微小RNA（miR）-29a-3p影响Serpinh1对胃癌细胞的增殖与侵袭,并分析其相关的分子机制。   方法  检测miR-29a-3p在胃癌BGC823细胞中的表达水平，并评估miR-29a-3p在胃癌细胞增殖、迁移和侵袭中的作用。通过Western blotting和荧光素酶分析证明，miR-29a-3p与Serpinh1 3’UTR直接结合。此外，采用MTT法、集落形成法和Transwell法在体外检测miR-29a-3p/Serpinh1轴对胃癌细胞增殖、迁移和侵袭的影响。  结果  转染后miR-29a-3p模拟物（mimic）组中miR-29a-3p的表达明显高于miR-29a-3p阴性对照和空白组，转染miR-29a-3p mimic后BGC823细胞增殖能力显著降低。荧光素酶分析显示，miR-29a-3p通过靶向Serpinh1 3’-非翻译区（3’UTR）抑制Serpinh1的表达。此外，miR-29a-3p的过表达通过靶向Serpinh1显著抑制胃癌细胞的增殖、侵袭和迁移。 
  结论  MiR-29a-3p可靶向作用于Serpinh1，从而调控胃癌细胞的增殖与侵袭。

关键词: 微小RNA-29a-3p, Serpinh1, 胃癌, 增殖, 侵袭, 免疫印迹法

Abstract:

Objective  To investigate the effects of microRNA(miR)-29a-3p on the proliferation and invasion of gastric cancer cells and analyze its related molecular mechanism.    Methods  The expression level of miR-29a-3p in gastric cancer cells was detected, and the role of miR-29a-3p in the proliferation, migration, and invasion of gastric cancer cells was evaluated. Western blotting and luciferase analysis showed that miR-29a-3p was directly bound to Serpinh1 3’-untranslated region(3’UTR). In addition, the effects of the miR-29a-3p/Serpinh1 axis on the proliferation, migration, and invasion of gastric cancer cells were detected by MTT assay, colony formation assay, and Transwell assay in vitro.   Results  After transfection, the expression of miR-29a-3p in the miR-29a-3p mimic group was significantly higher than that in the miR-29a-3p negative control and blank group. After transfection, the proliferation of BGC823 cells decreased significantly. Luciferase analysis showed that miR-29a-3p inhibited the expression of Serpinh1 by targeting the 3’UTR of Serpinh1. In addition, overexpression of miR-29a-3p significantly inhibited the proliferation, invasion, and migration of gastric cancer cells by targeting Serpinh1.  Conclusion  MiR-29a-3p can target Serpinh1 and regulate the proliferation and invasion of gastric cancer cells.

Key words: MicroRNA-29a-3p, Serpinh1, Gastric cancer, Proliferation, Invasion, Western blotting

中图分类号: