解剖学报

›› 2010, Vol. 41 ›› Issue (03): 446-451.doi: 10.3969/j.issn.0529-1356.2010.03.024

• 论著 • 上一篇    下一篇

大鼠大脑白质、皮质毛细血管的体视学定量研究

邵伟华1,2; 李琛１,2; 陈林1,2; 张伟1 ;唐勇1,2*   

  1. 1.重庆医科大学组织学与胚胎学教研室; 2.干细胞与组织工程学研究室，重庆 400016
  • 收稿日期:2009-04-02 修回日期:2009-05-11 出版日期:2010-06-06
  • 通讯作者: 唐勇

Stereological quantification of capillaries in rat white matter and cortex

  1. 1.Department of Histology and Embryology, Chongqing Medical University;2.Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China
  • Received:2009-04-02 Revised:2009-05-11 Online:2010-06-06
  • Contact: TANG Yong1

关键词: 皮质, 白质, 毛细血管, 免疫组织化学, 体视学, 大鼠

Abstract: Objective To study the total length, total volume, total surface area and average diameter of the capillaries in rat white matter and cortex. Methods In the present study, 5 adult LongEvans rats (7 months) were used. The length density, volume density, surface area density and total length, total volume, total surface area, average diameter of the capillaries in white matter and cortex were estimated with the stereological techniques and immunohistochemistry technique. Results The tissue processing induced volume shrinkage of rat white matter (±s) was 0.51 ± 0.09. The total length, total volume, total surface area and average diameter of the capillaries in the white matter was (30.9 ± 6.2) m，(0.8±0.09) mmSUP>3/SUP>，(5.3±0.74)cmSUP>2/SUP>，(5.5±1.1)μm. The tissue processing induced volume shrinkage of rat cortex was 0.57±0.08. The total length, total volume, total surface area and average diameter of the capillaries in the cortex was (364.8±68.0)m，(7.0±1.6)mmSUP>3/SUP>，(51.6±11.

Key words: Cortex, White matter, Capillary, Immunohistochemistry, Stereology, Rat

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