解剖学报

›› 2011, Vol. 42 ›› Issue (2): 155-158.doi: 10.3969/j.issn.0529-1356.2011.02.003

• 论著 • 上一篇    下一篇

低氧预适应后小鼠海马神经肽Y及突触的变化

曾宪智1*;沈慧娟2;王从荣1;许卫珍3; 郭燕君1;尹满香3   

  1. 1.嘉兴学院解剖学教研室，浙江 嘉兴314001;2.嘉兴学院机电工程中心，浙江 嘉兴314001;3.浙江省武警总医院病理科，浙江 嘉兴314000
  • 收稿日期:2010-08-02 修回日期:2010-09-09 出版日期:2011-04-06
  • 通讯作者: 曾宪智

Changes of neuropeptide Y and synapses in hippocampus after hypoxia preconditioning in mice

  1. 1.Department of Anatomy, College of Medicine, Jiaxing University, Zhejiang Jiaxing314001,China; 2.Laboratory Center of Engineering of Machinery and Electronics, Jiaxing University,Zhejiang Jiaxing314001,China; 3.General Hospital of Zhejiang Armed PAP Corps, Zhejiang Jiaxing314000,China
  • Received:2010-08-02 Revised:2010-09-09 Online:2011-04-06
  • Contact: ZENG Xian-zhi

关键词: 缺氧, 海马, 神经肽Y, 突触, 免疫组织化学, 小鼠

Abstract: ObjectiveTo explore the mechanism of neural protection after hypoxia preconditioning. MethodsMice were randomly divided into control group (H0 group) and hypoxia group (H4 group). H4 group were built up models of hypoxia preconditioning by keeping them in hypoxia repeatedly, H0 group were kept intact. The expression of neuropeptide Y (NPY) and synaptophysin in hippocampus was assessed with immunohistochemistry, and morphology of asymmetric synapses and perforated asymmetric synapses in the CA1 of hippocampus was observed by electron microscopy and their numbers were counted. ResultsComparing with H0 group, there was a moderate increase in the number of NPY positive cells,and a significant increase in the number of synaptophysin positive cells respectively in hippocampus(EM>n/EM>=30), but the number of asymmetric synapses and perforated asymmetric synapses presenting in CA1 region of hippocampus decreased in H4 group(EM>n/EM>=6). ConclusionThe changes took place in hippocampus after hypoxia preconditioning might decrease the neuronal exciting so that increase the brain’s tolerance to hypoxia/ischemia and thus protected neurons.

Key words: Hypoxia, Hippocampus, Neuropeptide Y, Synapse, Immunohistochemistry, Mouse

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