›› 2011, Vol. 42 ›› Issue (6): 741-745.doi: 10.3969/j.issn.0529-1356.2011.06.005

• 神经生物学 • 上一篇    下一篇

短暂缺氧对新生鼠脑细胞凋亡和神经再生的影响

李玉宇1,2; 徐剑文1*; 郭玮1; 刘晓艳1; 王玮1   

  1. 1.福建医科大学基础医学院人体解剖学与组织学胚胎学系,神经生物中心,福州 350004; 2.三明职业技术学院护理系,福建 三明 365000
  • 收稿日期:2011-02-16 修回日期:2011-04-06 出版日期:2011-12-06
  • 通讯作者: 徐剑文

Effects of transient hypoxia on apoptosis and neurogenesis in the newborn rat brain

  1. 1.Department of Anatomy and Histology and Embryology,College of Basic Medical Sciences,Center of Neurobiology, Fujian Medical University, Fuzhou 350004, China;2.Department of Nursing, Sanming Vocational Technical College,Fujian Sanming 365000, China
  • Received:2011-02-16 Revised:2011-04-06 Online:2011-12-06
  • Contact: XU Jian-wen

关键词: 缺氧, 神经源性分化因子, 神经发生, 免疫荧光, 原位缺口末端标记法, 免疫组织化学, 大鼠

Abstract: Objective To investigate effects of transient hypoxia on the expression level of neurogenic differentiation factor (NeuroD), apoptosis and neurogenesis in the rat brain.Methods The model was established as previously described by Grojean, newborn rats were transiently exposed to 100% N2. Ninety-six animals were randomly divided into normal control group and 20min hypoxia-treated group. Rats were sacrificed at day 13, 20 and 27 post-hypoxia, and the expression levels of NeuroD and BrdU in the brain were analyzed by immunofluorescence staining and immunohistochemistry. Alternatively, the rats were sacrificed at day 6 and 20 post-hypoxia, and apoptosis in the brain was analysed with TUNEL staining. Results Immunofluorescence/immunohistochemisty analysis showed that the expression of NeuroD and BrdU significantly increased at day 13 and 27 in the 20min hypoxia-treaded group compared to that in the control group (P0.05), with the highest expression level at day 20 (P0.01). TUNEL positive cells also significantly increased at day 6 in the 20min hypoxia-treaded group, while no difference was seen between the hypoxia-treated and the control group at day 20. Conclusion The transient neuronal loss induced by birth hypoxia may lead to cell proliferation, neuron differentiation and also may trigger neurogenesis, which may in turn participate in brain repai

Key words: Hypoxia, Neurogenic differentiation factor, Neurogenesis, Immunofluorescence, TUNEL, Immunohistochemistry, Rat

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