›› 2012, Vol. 43 ›› Issue (4): 545-548.doi: 10.3969/j.issn.0529-1356. 2012.04.019

• 组织学胚胎学发育生物学 • 上一篇    下一篇

部分肝切除后大鼠结肠黏膜层超微结构的变化

唐超智1; 张文学1; 高道文1; 张新胜1; 傅山岗1; 徐存拴1, 2*   

  1. 1.河南师范大学生命科学学院; 2.河南省-科技部共建细胞分化调控重点实验室, 河南 新乡 453007
  • 收稿日期:2011-05-27 修回日期:2011-07-10 出版日期:2012-08-06
  • 通讯作者: 徐存拴

Changes of the ultrastructure of colon mucosa after partial hepatectomy in rats

  1. 1.College of Life Sciences, He’nan Normal University, He’nan Xinxiang 453007, China;2.Co-Construction Key Laboratory for Cell Differentiation and Regulation, He’nan Xinxiang 453007, China
  • Received:2011-05-27 Revised:2011-07-10 Online:2012-08-06
  • Contact: XU Cun-shuan

关键词: 部分肝切除, 结肠, 黏膜层, 超微结构, 透射电镜, 大鼠

Abstract: Objective To study the effects of partial hepatectomy (PH) on the ultrastructure of colon mucosa in rats. Methods Thirty-five healthy SD rats were divided into one normal control group, three sham-operation (SO) control groups and three operation (2/3 PH) groups. The rats in the SO and PH groups were sacrificed at the 6th, 12th and 24th hour after speration). The colon was collected and prepared as ultrathin sections. Samples were observed by transmission electron microscope (TEM). Results Compared with that in the normal control group rats, the ultrastructure of colon mucosa did not show distinct changes in the SO groups. However, significant changes were observed in PH 6- and 12- hour groups rats, for example, the microvilli became short, enterocyte interspace was greatly increased, mitochondria of goblet cells was impaired, endocrine cells were stimulated, degranulation of mast cells was marked, macrophages were filled with phagosomes, neutrophilic granulocytes were gathered to lamina propria. In PH 24-hour groups, endocrine cells were still active, but other changes were not evident. Conclusion After PH, colon mucosa barrier is acutely damaged which leads a rising rate of infection. However, stimulation of endocrine cells and mast cells may be helpful to liver regeneration and recovery.

Key words: Partial hepatectomy, Colon, Mucosa, Ultrastructure, Transmission electron microscopy, Rat

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