解剖学报

›› 2012, Vol. 43 ›› Issue (6): 730-733.doi: 10.3969/j.issn.0529-1356.2012.06.002

• 神经生物学 •    下一篇

Nogo-p4对神经干细胞分化为双极形星形胶质细胞突起长度的影响

王丰*; 屠冠军; 朱悦   

  1. 中国医科大学附属第一医院骨科，沈阳 110001
  • 收稿日期:2012-03-02 修回日期:2012-04-16 出版日期:2012-12-06
  • 通讯作者: 王丰

Effect of Nogo-p4 on process growth of bipolar astrocyte differentiated from neural stem cells

  1. Department of Orthopedics, the First Affiliated Hospital, China Medical University, Shenyang 110001,China
  • Received:2012-03-02 Revised:2012-04-16 Online:2012-12-06
  • Contact: WANG Feng

摘要： 目的 观察Nogo-p4对大鼠脊髓来源神经干细胞（NSCs）分化形成双极形星形胶质细胞突起长度的影响。方法 取4只出生24h内的Wistar大鼠，悬浮培养法培养大鼠脊髓来源的NSCs，形成神经球后，以免疫荧光法鉴定NSCs。把NSCs分为A、B两组，A组加入血清，B组加入血清和Nogo-p4，分化7d后，胶质纤维酸性蛋白(GFAP)抗体标记星形

关键词: 神经干细胞, 星形胶质细胞, Nogo-p4, 悬浮培养法, 大鼠

Abstract: Objective To observe the effect of Nogo-p4 on process growth of bipolar astrocyte differentiated from neural stem cells (NSCs). Methods Neurospheres were collected by suspension culture of cells obtained from spinal cords of 4 Wistar rats born in 24 hours. Immunofluorescence method was used to identify NSCs and detect expression of glial fibrillary acidic portein (GFAP) after NSCs differentiated. The NSCs were divided into groups A and B. FBS was added to group A and FBS+Nogo-p4 was added to group B to initiate glial differentiation. The process length of bipolar astrocytes was measured. Results Seven days after differentiation, bipolar astrocytes were observed in both groups. The length of the process on bipolar astrocyte in group B was shorter than that in group A significantly. Conclusion The process growth of bipolar astrocytes differentiated from NSCs derived from the spinal cord of rats is inhibited significantly by Nogo-p4.

Key words: Neural stem cell, Astrocyte, Nogo-p4, Suspension culture, Rat

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