关键词: 乙酰葛根素, β-淀粉样肽, 阿尔茨海默病, 蛋白激酶C-δ, 白细胞介素-6, Morris水迷宫, 酶联免疫吸附试验, 大鼠

Abstract:

Objectiv To investigate the effect of acetylpuerarin on the expression of inflammation factors of protein kinase
C(PKC-δ) and interferon-6(IL-6) at the hippocampus stereotactic injection of Aβ1-42. Methods Forty Wistar rats were randomly
divided into the control group, AD model group,acetylpuerarin low-dose group and high-dose group. AD model rat was produced
by bilateral hippocampal injection of Aβ1-42.Learning and memory of the rat was tested through the method of Morris water
maze,ELISA was used to determine the expression of IL-6 and Western blotting to detect the changes of PKC-δ. Results Fourteen
days after Aβ1-42 injection, Morris water maze escape latency was more extended than pre-surgery and control group, and the
times of crossing the platform were significant reduction (P<0.01). After application acetylpueraria for 10 days, in the model
group the expression of IL-6 in the serum was significantly higher than the control group,acetylpuerarin low -dose group and
high-dose group (P<0.01); The amount of the expression of PKC-δ in the hippocampal homogenate of model group was significantly
higher than the control group, acetylpuerarin
low -dose group and high-dose group, but the expression of PKC-δ in the acetylpuerarin low -dose group and high-dose group was higher than the control group (P<0.05). Conclusion Acetylpuerarin can significantly improve the learning and memory ability of AD rats. It has obvious preventive and
protective effect on Alzheimer disease. The possible mechanism may be that acetylpueariu can regulate and control the Caspase pathway,attenuate the neurotoxicity of β-amyloid peptide, and reduce PKC-δ and IL-6 expression,and thus plays the role of anti-dementia.

Key words: Acetylpuerarin, Beta-amyloid peptide, Alzheimer disease, Protein kinase C-δ, Interleukin-6, Morris water maze, ELISA, Rat