解剖学报

解剖学报 ›› 2016, Vol. ›› Issue (2): 159-165.doi: 10.16098/j.issn.0529-1356.2016.02.003

• 神经生物学 • 上一篇    下一篇

不同张力扩张子宫颈诱导大鼠延髓内脏带c-Fos及神经型一氧化氮合酶的表达变化

沈良华1 吴仲敏2 郑春华3孙婷婷1 李卫云1 焦翠翠4陈新忠4凌树才1*   

  1. 1.浙江大学医学院解剖学教研室,杭州 310031; 2.台州学院医学院解剖学教研室，浙江 台州 318000; 3.台州医院门诊办公室，浙江 台州 317000; 4.浙江大学医学院附属妇产科医院, 杭州 310031
  • 收稿日期:2015-09-28 修回日期:2015-10-25 出版日期:2016-04-06 发布日期:2016-04-06
  • 通讯作者: 凌树才 E-mail:lingshucai@zju.edu.cn
  • 基金资助:

    代谢型谷氨酸受体1和5与瞬时受体电位香草酸受体通道1在子宫颈扩张相关的内脏痛中的作用

Expression changes of c-Fos and neuronal nitric oxide synthase in medullary visceral zone induced by varied distention of uterine cervicx

SHEN Liang-hua1 WU Zhong-min2 ZHENG Chun-hua3 SUN Ting-ting1 LI Wei-yun1 JIAO Cui-cui4 CHEN Xin-zhong4 LING Shu-cai 1*   

  1. 1. Department of Anatomy, School of Medicine, Zhejiang University，Hangzhou 310001， China; 2. Department of Anatomy, Medical College of Taizhou University，Zhejiang Taizhou 318000， China; 3.Womens Hospital, School of Medicine, Zhejiang University, Hangzhou 310001，China; 4.Outpatient Office, Taizhou Hospital, Zhejiang Taizhou 317000， China

  • Received:2015-09-28 Revised:2015-10-25 Online:2016-04-06 Published:2016-04-06
  • Contact: LING Shu-cai E-mail:lingshucai@zju.edu.cn

摘要：

Objective To investigate the expression changes of neuronal nitric oxide synthase (nNOS) and c-Fos in the rat medullary visceral zone with different uterine cervical distension(UCD),of which the transferring mechanism in the medulla oblongata is still unclear. Methods Adult female Sprague-Dawley rats were randomly divided into the control group (UCD 0g), 50g tension group (UCD 50g) and 100 g tension group (UCD 100g). The UCD model was established through distension forces generated by hanging different standard weights (50g,100g) at the end of metal crooks. The distribution of nNOS, c-Fos and c-Fos/nNOS positive neurons was observed 2 hours after UCD by NADPH histochemistry and c-Fos immunohistochemistry. The protein expression of nNOS and c-Fos in the medulla oblongata was detected by Western blotting. The mRNA expression of nNOS and c-Fos in the medulla oblongata was detected by Real-time PCR.
Results c-Fos immunoreactive neurons in nucleus were brown, round or oval, while the cytoplasm was unstained. The cell bodies and processes of nNOS positive neurons were blue, and the nuclei were not stained in the form of a bubble. c-Fos/nNOSdouble labeled cell bodies and dendrites of neurons stained blue, and nucleus showed brown yellow. These neurons were mainly distributed in the nucleus of the solitary tract nucleus (NTS) and the lateral reticular nucleus (LRN). Compared with UCD 0 g group, the number of nNOS, c-Fos and c-Fos/nNOS positive neurons in the NTS and LRN of UCD 50 g group and UCD 100 g group were significantly increased, and the dyeing significantly deepened. The protein and mRNA levels of c-Fos, nNOS in UCD 100 g group were higher than that of UCD 50 g group (P<0.05). And both of the two groups were significantly elevated (P<0.01) as compared with UCD 0 g group. Conclusion Acute uterine cervical dilatation in rats can lead to increased expression of c-Fos and nNOS in NTS and LRN of the medulla oblongata, nNOS positive neurons in NTS and LRN may be involved in the conduction of the UCD pain in the medulla oblongata.

Abstract:

Objective To investigate the expression changes of neuronal nitric oxide synthase (nNOS) and c-Fos in the rat medullary visceral zone with different uterine cervical distension(UCD),of which the transferring mechanism in the medulla oblongata is still unclear. Methods Adult female Sprague-Dawley rats were randomly divided into the control group (UCD 0g), 50g tension group (UCD 50g) and 100 g tension group (UCD 100g). The UCD model was established through distension forces generated by hanging different standard weights (50g,100g) at the end of metal crooks. The distribution of nNOS, c-Fos and c-Fos/nNOS positive neurons was observed 2 hours after UCD by NADPH histochemistry and c-Fos immunohistochemistry. The protein expression of nNOS and c-Fos in the medulla oblongata was detected by Western blotting. The mRNA expression of nNOS and c-Fos in the medulla oblongata was detected by Real-time PCR.
Results c-Fos immunoreactive neurons in nucleus were brown, round or oval, while the cytoplasm was unstained. The cell bodies and processes of nNOS positive neurons were blue, and the nuclei were not stained in the form of a bubble. c-Fos/nNOSdouble labeled cell bodies and dendrites of neurons stained blue, and nucleus showed brown yellow. These neurons were mainly distributed in the nucleus of the solitary tract nucleus (NTS) and the lateral reticular nucleus (LRN). Compared with UCD 0 g group, the number of nNOS, c-Fos and c-Fos/nNOS positive neurons in the NTS and LRN of UCD 50 g group and UCD 100 g group were significantly increased, and the dyeing significantly deepened. The protein and mRNA levels of c-Fos, nNOS in UCD 100 g group were higher than that of UCD 50 g group (P<0.05). And both of the two groups were significantly elevated (P<0.01) as compared with UCD 0 g group. Conclusion Acute uterine cervical dilatation in rats can lead to increased expression of c-Fos and nNOS in NTS and LRN of the medulla oblongata, nNOS positive neurons in NTS and LRN may be involved in the conduction of the UCD pain in the medulla oblongata.