解剖学报

›› 2016, Vol. 47 ›› Issue (1): 117-121.

• 组织学胚胎学发育生物学 • 上一篇    下一篇

树突状细胞在子宫内膜异位症内膜组织中的变化

魏静波   

  1. 华北理工大学基础医学院组织胚胎学系
  • 收稿日期:2015-08-14 修回日期:2015-10-30 出版日期:2016-02-06 发布日期:2016-02-06
  • 通讯作者: 魏静波 E-mail:weijingbo@sohu.com
  • 基金资助:
    河北省科技厅科研基金资助项目;河北省科技支撑项目;唐山市科技局科研基金资助项目

Changes of dendritic cells in endometrial tissue of endometriosis

  • Received:2015-08-14 Revised:2015-10-30 Online:2016-02-06 Published:2016-02-06

摘要： 目的 检测不成熟DCs及成熟DCs的标记物CD1a和CD83在子宫内膜异位症（EMS）内膜组织中的表达，探讨不同发育阶段的DCs在子宫内膜异位症发生发展中的作用。方法 选取病理检查确诊 的“巧克力囊肿”患者30例，分别取其异位内膜组织（异位内膜组）和在位内膜组织（在位内膜组），同时选取30例非内异症内膜组织为对照组。免疫组织化学法检测CD1a、CD83蛋白表达和分布，免疫印记法检测CD1a、CD83蛋白表达量。结果 CD1a和CD83阳性细胞均分布于固有层基质细胞间，血管周围；CD1a蛋白在对照组、在位内膜组、异位内膜组表达依次升高，组间比较差异均有统计学意义(P<0.01)；CD83蛋白在对照组、在位内膜组、异位内膜组表达依次减少，差异均有统计学意义(P<0.01)。结论 子宫内膜异位症患者子宫内膜局部imDCs增多、mDCs减少，可能在子宫内膜异位症中发挥作用。

关键词: 子宫内膜异位症, 树突状细胞, 免疫排斥, 免疫耐受, CD1a, CD83

Abstract: Objective　To investigate the expression of CD1a and CD83 which were themarkers of immature dendritic cells (imDCs) and mature dendritic cells (mDCs) respectively in endometrial tissue of endometriosis, and explore the role of DCs at different stages in the occurrence and development of endometriosis.　Methods　30 cases with chocolate cyst in the proliferative phase confirmed by pathological examination were selected, the ectopic endometrium tissues and the ectopic endometrium tissues were taken as eutopic endometrium of EMS group, ectopic endometrium of EMS group EMS and in the endometrium, another 30 cases of normal endometrial tissue were taken as control group. Immunohistochemical straining were used to detect the location and the expressions of CD1a and CD83, and western blotting were used to detect the expressions of CD1a and CD83 　Results　The CD1a and CD83 positive cells are scattered among stromal cells; The expression of CD1a increased in control group, ectopic endometrium group and eutopic endometrium group, and the difference between groups was significant (P<0.01); the expression of CD83 decreased in control group, ectopic endometrium group and eutopic endometrium group, and the difference between groups was significant (P<0.01). Conclusion Compared with non EMS patients, the increase of imDCs and the decrease of mDCs in local endometrium in endometriosis may play important roles in the occurrence and development of EMS.

Key words: Endometriosis, dendritic cells, Immune response, Immune tolerance, CD1a, CD83