高尔基体磷蛋白3在结直肠癌组织中表达及其对结直肠癌细胞增殖、侵袭和迁移的影响

doi:10.16098/j.issn.0529-1356.2018.04.008

摘要/Abstract

摘要：

目的分析高尔基体磷蛋白3（GOLPH3）在结直肠癌组织中的表达情况，及其对结直肠癌细胞增殖、侵袭和迁移的影响。方法收集术前未经放、化疗治疗，经手术切除患者的结直肠癌组织及其相应的癌旁组织（40例）；采用免疫组织化学法检测组织中GOLPH3的表达，分析GOLPH3与结直肠癌临床病理特征的关系；构建稳定沉默GOLPH3的结直肠癌细胞株，并设立阴性对照组和空白对照组，Western blotting法检测3组细胞中GOLPH3蛋白的表达，MTT法检测3组细胞的增殖活性，Transwell实验分别检测3组细胞的侵袭和迁移能力。结果 GOLPH3在癌组织中主要为阳性表达，且阳性表达率（65.0%）显著高于癌旁组织（35.0%）（P＜0.05）；GOLPH3在TNM分期为Ⅲ～Ⅳ期、浸润深度≥2 cm、中低分化及有淋巴结转移的癌组织中的阳性表达率明显高于TNM分期Ⅰ～Ⅱ期、浸润深度＜2 cm、高分化及无淋巴结转移的癌组织（P＜0.05）。与阴性对照组和空白对照组比，基因沉默组GOLPH3蛋白表达量、细胞增殖活性、侵袭能力及迁移能力均明显降低（P＜0.05）。结论 GOLPH3在结直肠癌组织中高表达，其表达与肿瘤分期、侵袭深度、分化程度及淋巴结转移有关，抑制GOLPH3基因表达可下调GOLPH3蛋白表达，抑制结直肠癌细胞增殖、侵袭和迁移。

关键词: 结直肠癌, 高尔基体磷蛋白3, 基因沉默, 细胞增殖, 免疫组织化学, 人

Abstract:

Objective To analyze the expression of Golgi phosphoprotein 3 (GOLPH3) in colorectal cancer tissues and its influence on the proliferation, invasion and migration of colorectal cancer cells. Methods The colorectal cancer tissues and paracancerous tissues were collected from the surgically resected colorectal cancer patients (40 cases) without radiotherapy or chemotherapy before the operation. The expression of GOLPH3 in the colorectal cancer tissues and paracancerous tissues were detected by immunohistochemistry, and the relationship between GOLPH3 and clinicopathological features of colorectal cancer was analyzed. Colorectal cancer cell starin with GOLPH3 stable silent was constructed, and a negative control group and blank control group were established. The expression of GOLPH3 protein of cells in the three groups was detected by Western blotting. MTT assay was used to detect the proliferative activity of cells in the three groups. The invasion ability and migration ability of cells in the three groups were detected by transwell test. Results The expression of GOLPH3 was mainly positive in the cancer tissue sample, and the positive expression rate (65.0%) of the cancer tissues was significantly higher than that of the paracancerous tissues (35.0%) (P<0.05). The GOLPH3 positive expression rate of cancer tissues with TNM stage Ⅲ-Ⅳ, invasion depth≥2 cm, middle-low differentiation and lymphatic metastasis were significantly higher than that of cancer tissues with TNM stageⅠ-Ⅱ, invasion depth<2 cm and high differentiation and without lymphatic metastasis (P<0.05). Compared with the negative control group and the blank control group, the expression level of GOLPH3, the cell proliferation activity, the invasion ability and migration ability rate in the gene silencing group were significantly lower (P<0.05). Conclusion GOLPH3 is highly expressed in the colorectal cancer tissues, and the expression level is related to the tumor staging, invasion depth, differentiation and lymphatic metastasis. Inhibiting the expression of GOLPH3 gene could deregulate the GOLPH3 protein level, and inhibit the proliferation, invasion and migration of colorectal cancer cells.

Key words: Colorectal cancer, Golgi phosphoprotein 3, Gene silencing, Cell proliferation, Immunohistochemistry, Human

刘师宏汪威穆小松. 高尔基体磷蛋白3在结直肠癌组织中表达及其对结直肠癌细胞增殖、侵袭和迁移的影响[J]. 解剖学报, 2018, 49(4): 461-468.

LIU Shi-hong WANG Wei MU Xiao-song. Expression of Golgi phosphoprotein 3 in colorectal cancer tissues and its effects on proliferation, invasion and migration of colorectal cancer cells[J]. Acta Anatomica Sinica, 2018, 49(4): 461-468.

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