解剖学报

解剖学报 ›› 2018, Vol. 49 ›› Issue (4): 512-517.doi: 10.16098/j.issn.0529-1356.2018.04.016

• 组织学胚胎学发育生物学 • 上一篇    下一篇

着丝粒蛋白A在大鼠心脏的表达及增龄变化

冯颖 殷国田 李爱荣* 郭志坤*   

  1. 新乡医学院 河南省医用组织再生重点实验室，河南 新乡 453003
  • 收稿日期:2017-11-07 修回日期:2018-01-30 出版日期:2018-08-06 发布日期:2018-08-06
  • 通讯作者: 李爱荣;郭志坤 E-mail:gzk@xxmu.edu.cn

Expression and age-related changes of centromere A in rat heart

FENG Ying YIN Guo-tian LI Ai-rong* GUO Zhi-kun*   

  1. He’nan Key Laboratory of Medical Tissue Regeneration，Xinxaing Medical University, He’nan Xinxiang 453003, China
  • Received:2017-11-07 Revised:2018-01-30 Online:2018-08-06 Published:2018-08-06
  • Contact: Ai-rong;GUO Zhi-kun E-mail:gzk@xxmu.edu.cn

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摘要：

目的  探讨着丝粒蛋白A（CENP-A）在大鼠心脏中的表达部位及增龄变化。方法  取新生1 d、1周龄、2周龄、3周龄、4周龄、3月龄、6月龄大鼠各10只，采用免疫组织化学、免疫荧光、Western blotting、Real-time PCR技术，观察心脏不同部位CENP-A的分布和增龄变化；利用图像分析系统对CENP-A进行定量分析，并对不同年龄组心脏CENP-A含量进行对比。结果  大鼠心脏中CENP-A的表达主要分布在血管壁、心外膜及外膜下组织。随着年龄增长血管壁中CENP-A表达基本稳定，心外膜及外膜下组织中CENP-A表达逐渐减少，新生1 d表达最多，3月龄、6月龄明显减少。血管壁中CENP-A主要是在平滑肌细胞表达，且各年龄段表达基本不变。在靠近心外膜的组织中也有少量心肌细胞表达，且随增龄变化而减少。结论  大鼠心脏CENP-A的表达随增龄变化逐渐减少，血管壁CENP-A的表达稳定不变。

关键词: 着丝粒蛋白, 心肌组织, 心外膜, 心脏, 免疫组织化学, 免疫荧光, 大鼠

Abstract:

Objective  To study the expression and age-related changes of centromere protein A(CENP-A) in the rat heart. Methods  Ten rats in each group were collected from postnatal 1 day, 1 week old, 2 weeks old, 3 weeks old, 4 weeks old, 3 months old, 6 months old. The expression and age-related changes of CENP-A in the rat heart were observed by the immunohistochemistry, immunofluorescence, Western blotting, and Real-time PCR techniques. The CENP-A proteins of seven groups were quantitated by SPSS 20.0 statistical software. Results  CENP-A was mainly expressed in the vascular wall, epicardium and epicardial tissue and in a very few cardiomyocytes. The CENP-A in vascular wall was of stable expression with age. But the expression of CENP-A in the epicardium and subepicardial tissues was gradually reduced, which showed that postnatal 1 day was the most and 6 months old are the least. CENP-A in the vascular wall was mainly expressed in the smooth muscle cells, and was basically unchanged with ages.A small number of cardiac myocytes near the subepicardium also express CENP-A, and decreased with age. Conclusion  The expression of CENP-A in the rat heart gradually decreased with aging, but in the vascular wall remained stable expression.

Key words: Centromere protein-A, Myocardial tissue, Epicardium, Heart, Immunohistochemistry, Immunofluorescence, Rat