解剖学报

解剖学报 ›› 2018, Vol. 49 ›› Issue (5): 579-583.doi: 10.16098/j.issn.0529-1356.2018.05.003

• 神经生物学 • 上一篇    下一篇

MicroRNA-613在人胶质瘤中的表达及其对增殖与转移的影响

李传芬1,5荆文2 向冬生3 李涛4 焦淑欣5 杨翠玲5 马志红5 王敏1*   

  1. 1. 山东师范大学生命科学学院，济南 250014; 2. 山东师范大学体育学院， 济南 250014; 3. 山东职业学院电气系，济南 250104;  4. 临沂市疾病预防控制中心微生物检验科，山东 临沂 276000; 5. 济南军区总医院神经内科，济南 250031
  • 收稿日期:2018-03-22 修回日期:2018-04-04 出版日期:2018-10-06 发布日期:2018-10-06
  • 通讯作者: 王敏 E-mail:lcf23598@163.com
  • 基金资助:
    山东省科技重点研发项目（2016GSF201058）；山东省自然科学基金青年基金资助项目（ZR2014CQ026）

Expression of microRNA-613 in human glioma and its effects on growth and metastasis

LI Chuan-fen 1,5 JING Wen2 XIANG Dong-sheng3 LI Tao4 JIAO Shu-xin5 YANG Cui-ling5 MA Zhi-hong5 WANG Min 1*   

  1. 1. College of Life Sciences,  Shandong Normal University, Ji’nan 250014, China; 2. College of Physical Education,  Shandong Normal University, Ji’nan 250014, China; 3. Electrical Department, Shandong Vocational College, Ji’nan 250104, China;  4. Department of Microbiology Laboratory, Linyi Disease Prevention and Control Center, Shandong Linyi 276000, China; 5. Department of Neurology, General Hospital of Ji’nan Military Region, Ji’nan 250031, China
  • Received:2018-03-22 Revised:2018-04-04 Online:2018-10-06 Published:2018-10-06
  • Contact: WANG Min E-mail:lcf23598@163.com

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摘要：

目的 探讨microRNA-613(miR-613)在人胶质瘤中的表达及其对增殖与转移的影响。 方法 采用Real-time PCR法对30例人胶质瘤患者瘤组织和30例非胶质瘤患者的脑组织的miR-613表达水平进行检测，并分析其与胶质瘤临床病理特征及预后的关系；采用LipofectamineTM2000将miR-613模拟物（miR-613 mimics）和无关序列对照（miR-613 con）转入U87细胞中，Real-time PCR检测两组细胞miR-613的表达水平，CCK8法检测细胞增殖变化，Transwell实验检测细胞侵袭和迁移能力的变化。 结果 miR-613在胶质瘤组织中表达低于正常的脑组织（P<0.05）。胶质瘤组织miR-613的表达水平与病理分级成负相关，与生存时间成正相关（P<0.05）；与miR-613 con比较，miR-613 mimics细胞增殖水平、侵袭与迁移能力显著降低（P<0.05）。 结论 miR-613具有抑制胶质瘤细胞的生长、侵袭与迁移的作用，有望成为胶质瘤的潜在治疗靶点。

关键词: microRNA-613, 胶质瘤, U87, 生长, 转移, 实时定量聚合酶链反应, 人

Abstract:

Objective To investigate the expression of microRNA-613(miR-613) in human glioma and its effect on the proliferation and metastasis. Methods Thirty human glioma patients tissues and 30 Non-glioma patients tissueswere detected by Real-time PCR, and to analysie their relationship with clinicopathological characteristics of gliomas. MiR-613mimics and unrelated sequence control(miR-613 con) were transfected into U87 cells using Lipofectamine TM2000. The expression of miR-613 in the two groups of cells was detected by Real-time PCR, the proliferation of cells was detected by CCK8 assay, and the invasion and migration of cells were detected by Transwell assay. Results The expression of miR-613 in Glioma tissues was lower than that in corresponding paracancerous tissues (P<0.05). The expression level of miR-613 in glioma tissue was negatively correlated with pathological grade and positively correlated with survival time (P<0.05). Compared with miR-613 con cells, miR-613 mimics cells proliferation, invasion and migration were significantly decreased (P<0.05). Conclusion MiR-613 can inhibit the proliferation, invasion and migration of glioma cells, and it might be a potential therapeutic target for the treatment of glioma.

Key words: MicroRNA-613, Glioma, U87, Growth, Metastasis, Real-time PCR, Human