调控内侧前额叶皮质向丘脑室旁核投射通路对小鼠痛信息传递的影响

doi:10.16098/j.issn.0529-1356.2024.04.008

解剖学报 ›› 2024, Vol. 55 ›› Issue (4): 430-436.doi: 10.16098/j.issn.0529-1356.2024.04.008

调控内侧前额叶皮质向丘脑室旁核投射通路对小鼠痛信息传递的影响

朱柯桦^1,2吴凤玲²孙寒雪²洪洁²陈思海²史娟^2*李云庆^2*

1.西北工业大学医学研究院，西安 710072; 2.空军军医大学基础医学院人体解剖学与组织学胚胎学教研室，暨梁銶琚脑研究中心，西安 710032

收稿日期:2024-02-05 修回日期:2024-03-14 出版日期:2024-08-06 发布日期:2024-08-06
通讯作者: 史娟李云庆 E-mail:deptanat@fmmu.edu.cn

Effect of modulating the pathway from the medial prefrontal cortex to the thalamic paraventricular nucleus on pain transmission in mice

ZHU Ke-hua^1,2 WU Feng-ling²SUN Han-xue²HONG Jie² CHEN Si-hai²SHI Juan^2* LI Yun-qing^2*

1.Institute of Medical Research, Northwestern Polytechnical University, Xi’an 710072, China; 2.Department of Anatomy, Histology and Embryology, K. K. Leung Brain Research Centre, School of Basic Medicine, Air Force Military Medical University, Xi’an 710032, China

Received:2024-02-05 Revised:2024-03-14 Online:2024-08-06 Published:2024-08-06
Contact: SHI Juan LI Yun-qing E-mail:deptanat@fmmu.edu.cn

摘要/Abstract

摘要：

目的探讨小鼠内侧前额叶皮质（mPFC）-丘脑室旁核（PVT）通路的投射神经元性质及调控该通路对生理痛和急性痛的影响。方法将CTb注入GAD67-GFP转基因小鼠的PVT内，观察向PVT投射的mPFC神经元的性质，使用化学遗传学方法调控mPFC-PVT通路，观察对小鼠机械痛、热痛和冷痛以及辣椒素诱导的急性炎性痛的影响。结果 mPFC内CTb标记神经元主要分布于V层和VI层且与GAD67-GFP不共标；化学遗传学方法激活mPFC-PVT通路可显著降低小鼠的机械痛阈值（p < 0.0001），缩小热痛潜伏期（p < 0.001），对冷痛无明显的影响，抑制此通路则可显著提高动物的机械性痛阈（p < 0.05）；辣椒素诱导急性炎性痛模型下激活此通路引起小鼠舔爪时间增加（p < 0.05）。结论 mPFC-PVT通路为非GABA能的神经通路，激活该通路可促进小鼠的机械痛、热痛、急性炎性痛反应。

关键词: 内侧前额叶皮质, 丘脑室旁核, 生理痛, 急性炎性痛, 化学遗传学, 小鼠

Abstract:

Objective To explore the neuronal properties of the pathway from the medial prefrontal cortex (mPFC) to the paraventricular thalamic nucleus of (PVT) and to investigate the effect of modulation of the pathway on physiological pain and acute pain in mice. Methods CTb was injected into the PVT of GAD67-GFP transgenic mice, and the properties of mPFC neurons projected to PVT were observed. The mPFC-PVT pathway was activated or inhibited by chemogenetics to observe the effects on physiological pain, such as mechanical pain, thermal pain, cold pain, and on capsaicin induced inflammatory pain. Results CTB-labeled neurons in the mPFC were mainly distributed in layer V and layer VI, and were not double-labeled with GAD67-GFP. Chemogenetic activation of the mPFC-PVT pathway significantly decreased the mechanical pain threshold (p < 0.0001) and shortened the thermal pain latency (p < 0.001), but had no obvious effects on cold pain. Inhibition of this pathway significantly increased the mechanical pain threshold (p < 0.05). Activation of the pathway increased the paw licking time (p < 0.05) in acute inflammatory pain induced by intraplantar injection of capsaicin. Conclusion mPFC-PVT pathway is a non GABAergic projection and its activation can promote mechanical pain, thermal pain, and acute inflammatory pain in mice.

Key words: Medial prefrontal cortex, Thalamic paraventricular nucleus, Physiological pain, Acute inflammatory pain, Chemogenetics, Mouse

中图分类号:

R322

朱柯桦吴凤玲孙寒雪洪洁陈思海史娟李云庆. 调控内侧前额叶皮质向丘脑室旁核投射通路对小鼠痛信息传递的影响[J]. 解剖学报, 2024, 55(4): 430-436.

ZHU Ke-hua WU Feng-ling SUN Han-xue HONG Jie CHEN Si-hai SHI Juan LI Yun-qing. Effect of modulating the pathway from the medial prefrontal cortex to the thalamic paraventricular nucleus on pain transmission in mice[J]. Acta Anatomica Sinica, 2024, 55(4): 430-436.

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调控内侧前额叶皮质向丘脑室旁核投射通路对小鼠痛信息传递的影响

Effect of modulating the pathway from the medial prefrontal cortex to the thalamic paraventricular nucleus on pain transmission in mice

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