解剖学报

›› 2006, Vol. 37 ›› Issue (6): 605-610.doi:

• 论著 •    下一篇

外周注射福尔马林诱导大鼠前扣带皮层中ERK-CREB的激活

高永静1,2;赵志奇2;张玉秋2*   

  1. 1.南通大学航海医学研究所，南通 226001;2.复旦大学神经生物学研究所，上海 200433
  • 收稿日期:2005-12-27 修回日期:2006-04-17 出版日期:2006-12-06
  • 通讯作者: 张玉秋

The Activation of Extracellular Signal Regulated Kinase and cAMP Respo nsive Element Binding Protein in the Anterior Cingulte Cortex Induced by Perip heral Injection of Formalin

  1. 1.Institute of Nautical Medicine,Nantong University, Nantong 226001,China;2Institute of Neurobiology, Fudan University, Shanghai 200433,China
  • Received:2005-12-27 Revised:2006-04-17 Online:2006-12-06
  • Contact: ZHANG Yu-qiu

关键词: 前扣带皮层, 细胞外信号调节激酶, cAMP反应元件结合蛋白, 疼痛:免疫组织化学, 免疫蛋白印迹, 大鼠

Abstract: Objective To investigate the activation of extracellular signalregulated kinase (ERK) an d cAMPresponsive element binding protein (CREB) in the anterior cingulate cortex (ACC) induced by peripheral injection of formalin. Methods 5% formalin was injected subcutaneously into the unilateral hindpaw. The rats w BR>ere perfused or killed and the ACC dissected at different time points. The activation of phosphorylated ERK (pERK) and phosphorylated CREB (pCREB) in the ACC we are observed by immunohistochemical and Western blotting techniques. Results Subcutaneous injection of formalin induced the activation of pCREB and pERK in the ACC. Formalininduced the activation of pERK and pCREB in the ACC peaked at 3 min and 30 min after stimulation, respectively. The total ERK and CREB had no significant changes. Conclusion The ACC could receive peripheral noxious information and the ERK and CREB in the BR>ACC might involve in the processing of the primary unpleasentn

Key words: Anterior cingulate cortex, Extracellular signalregulated kinase, cAMP responsi BR>ve element binding protein, Pain, Immunohistochemistry, Western blotting, Rat