嘌呤能离子通道型受体7介导的帕金森大鼠认知功能障碍

doi:10.16098/j.issn.0529-1356.2023.05.005

摘要/Abstract

摘要：

目的探讨嘌呤能离子通道型受体7（P2X7R）在帕金森（PD）大鼠认知功能障碍中的作用。方法成年大鼠90只实验分为5组，每组6只大鼠，3个重复，运用6-羟基多巴胺(6-OHDA)制备PD大鼠模型。向PD大鼠注射P2X7R激动剂2,3-腺苷5-三磷酸三乙铵盐（BzATP)和拮抗剂考马斯亮蓝G(CBBG)检测大鼠在水迷宫中的学习记忆能力和疼痛反应，Real-time PCR和Western blotting检测海马组织P2X7的表达情况。结果与正常大鼠相比，PD大鼠学习速度缓慢，记忆能力弱，CBBG提高了PD大鼠的学习记忆能力，BzATP使PD大鼠的学习记忆能力减退；海马组织Real-time PCR结果显示，与对照组相比，P2X7R在海马组织中mRNA表达量最高，CBBG组P2X7R表达量下调，BzATP组P2X7R表达量上调；对P2X7R进行Western blotting检测，与PD组相比，BzATP组P2X7蛋白表达量显著升高，CBBG组P2X7蛋白表达量较低。结论 PD大鼠存在认知功能障碍，CBBG可改善PD大鼠的学习记忆功能，BzATP可抑制PD大鼠的学习记忆功能。

关键词: 帕金森病, 认知功能, 嘌呤能离子通道型受体, 2, 3-腺苷5-三磷酸三乙铵盐受体, 免疫印迹法, 大鼠

Abstract:

Objective To explore the role of purinergic ligand-gated ion chennel 7 receptor(P2X7R) in cognitive dysfunction in Parkinson’s disease rats. Methods Ninty rats was divided into 5 groups with 6 rats in each group with 3 repeats. The rat model of PD was established by 6-hydroxydopamine (6-OHDA). PD rats were injected with P2X7R agonists 2,3-adenosine 5-triethylammonium triphosphate (BzATP) and antagonists Comassie brilliant blue G (CBBG). The learning and memory ability and pain response of rats in the water maze were measured, and the expression of P2X7 in hippocampus was detected by Real-time PCR and Western blotting. Results Compared with the normal rats, PD rats had slow learning speed and weak memory ability. CBBG improved the learning and memory ability of PD rats, while BzATP decreased the learning and memory ability of PD rats. The result of Real-time PCR showed that compared with the control group, the expression of P2X7R mRNA was the highest in hippocampal tissue, the expression of P2X7R in CBBG group was down-regulated, and that of P2X7R in BzATP group was up-regulated. Compared with the PD group, Western blotting of P2X7R showed that the expression of P2X7 protein increased significantly in BzATP group, while the expression of P2X7 protein was lower in CBBG group. Conclusion Cognitive impairment exists in PD rats. CBBG can improve the learning and memory function of PD rats, and BzATP can inhibit the learning and memory function of PD rats.

Key words:

Parkinson’s disease, Cognitive function, Purinergic ligand-gated ion channel 7 receptor;2,3-adenosine 5-triethylammonium triphosphate receptor, Western blotting, Rat

中图分类号:

R-332

燕洋洋郭凯孙志宏 . 嘌呤能离子通道型受体7介导的帕金森大鼠认知功能障碍[J]. 解剖学报, 2023, 54(5): 531-537.

YAN Yang-yang GUO Kai SUN Zhi-hong . Purinergic ligand-gated ion channel 7 receptor-mediated cognitive impairment in Parkinson’s disease rats[J]. Acta Anatomica Sinica, 2023, 54(5): 531-537.

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