解剖学报

›› 2012, Vol. 43 ›› Issue (5): 614-618.doi: 10.3969/j.issn.0529-1356.2012.05.006

• 细胞和分子生物学 • 上一篇    下一篇

炎性环境下内皮微粒介导单核细胞促内皮细胞增殖的作用

王雪颖1; 王世坤1; 田铧1* ; 索宁1; 房云海2; 魏玉梅3; 扈燕来1; 宋涛1   

  1. 1.山东大学医学院解剖学与组织学胚胎学研究所，济南 250012; 2.山东省血液中心血友病研究所，济南 250010；3.山东省肿瘤医院放射科，济南 250010
  • 收稿日期:2012-01-20 修回日期:2012-06-01 出版日期:2012-10-06
  • 通讯作者: 田铧

Effect of endothelial microparticles on monocytes to promote endothelial cell proliferation in inflammation

  1. 1. Institute of Anatomy and Histology and Embryology, Medical School of Shandong University, Ji′nan 250012, China; 2. Hemophilia Institute of Blood Center of Shandong Province, Ji′nan 250010,China; 3. Department of Radiation Oncology Shandong Cancer Hospital, Ji′nan 250010, China
  • Received:2012-01-20 Revised:2012-06-01 Online:2012-10-06
  • Contact: TIAN Hua

关键词: 炎性因子, 内皮微粒, 单核细胞, 脐静脉内皮细胞, 免疫荧光, 酶联免疫吸附法, 透射电镜, 人

Abstract: Objective To investigate the effect of endothelial microparticles (EMPs) on monocytes in promoting inflammatory angiogenesis and in expressing endothelial markers. Methods EMPs were isolated from the supernatants of cultured human umbilical vein endothelial cells (HUVECs) treated with TNF-αby ultracentrifugation, identified under an electron microscope and applied to cultured THP-1 cells. The level of VEGF-A and VEGF-C expressed by THP-1 cells was examined with ELISA. The expression of von Willebrand factor (vWF) and vascular endothelial growth factor receptor 2 (VEGFR-2) in THP-1 cells were studied with RT-PCR and immunofluorescence. HUVECs were co-cultured with THP-1 cells either treated with EMPs not. The proliferation of HUVECs was assessed. Results TNF-α enhanced HUVECs to release EMPs, which promoted THP-1 cells to secrete vascular endothelial growth factor A (VEGF-A) and vascular endothelial growth factor C (VEGF-C). THP-1 cells did not express vWF or VEGFR-2 genetically after either 3 days or 8 days of culture. When cultured with THP-1 cells, HUVECs were more active in proliferation. Conclusion EMPs are able to activate monocytes which in turn enhanced endothelial cells growth by secreting

Key words: Inflammatory factor, Endothelial microparticle, Monocyte, Umbilical vascular endothelial cell, Immunofluorescence, ELISA, Transmission electron microscopy, Human

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