›› 2012, Vol. 43 ›› Issue (6): 864-867.doi: 10.3969/j.issn.0529-1356.2012.06.027
• 技术方法 • 上一篇 下一篇
杨世明; 李和*
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摘要: 目的 探索一种以正丁醇替代无水乙醇和二甲苯制作不同胎龄大鼠全胚切片的简便方法。方法 收集胚龄14d(E14)、E16、E18、E20大鼠胚胎,固定及低浓度乙醇脱水后,部分标本按传统方法用无水乙醇脱水和二甲苯透明(无水乙醇-二甲苯法),另一部分标本用正丁醇脱水、透明(正丁醇法)。所有标本进行石蜡包埋、切片、H
关键词: 全胚切片, 正丁醇, 无水乙醇, 二甲苯, 苏木素-伊红染色, 大鼠
Abstract: Objective To explore a simple method to replace absolute ethanol and xylene with n-butanol for preparation of sections of whole rat embryos at different gastational ages. Methods Embryonic day (E) 14, 16, 18, 20 rat embryos were collected and fixed. After dehydration in low concentrations of the ethanol, some embryos were treated with the traditional absolute ethanol-xylene method in which absolute ethanol and xylene were used for dehydration and clearing, and other embryos were further dehydrated and cleared with the n-butanol method. Then, all the embryos were embedded in paraffin, cut on a microtome, and stained with hematoxylin-eosin. The quality of embedded blocks and embryo sections was compared and evaluated. Results Compared with the absolute ethanol-xylene method for preparation of whole rat embryo sections, the n-butanol method was more simple, and easier to control the time in the whole processes. Moreover, the embryos treated with n-butanol had better cutting performance and higher quality in morphological preservation of the hepatic tissue. Conclusion As a simple, safe and reliable method, the n-butanol method can replace the absolute ethanol-xylene method for preparation of whole rat embryo sections.
Key words: Whole embryo section, N-butanol, Absolute ethanol, Xylene, Hematoxylin-eosin staining, Rat
中图分类号:
Q954.5
杨世明;李和. 正丁醇替代无水乙醇和二甲苯在制作大鼠全胚切片中的应用 [J]. , 2012, 43(6): 864-867.
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链接本文: https://jpxb.bjmu.edu.cn/CN/10.3969/j.issn.0529-1356.2012.06.027
https://jpxb.bjmu.edu.cn/CN/Y2012/V43/I6/864
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