解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (1 ): 44-48.

• 细胞和分子生物学 • 上一篇    下一篇

辅酶Q10对大鼠再生肝细胞线粒体通透性转换的影响

翟心慧1,来小海1,吴清华1,赵爱景1,徐存拴1,2*   

  1. 1.河南师范大学生命科学学院;2.河南省-科技部共建细胞分化调控重点实验室，河南 新乡 453007
  • 收稿日期:2012-03-01 修回日期:2012-05-07 出版日期:2013-02-06 发布日期:2013-02-06
  • 通讯作者: 徐存拴 E-mail:xucs@x263.net.cn

Effects of coenzyme Q10 on mitochondrial permeability transition of regenerating hepatocytes in rats

ZHAI Xin-hui1, LAI Xiao-hai1, WU Qing-hua1,ZHAO Ai-jing1, XU Cun-shuan 1,2 *   

  1. 1.College of Life Sciences, He’nan Normal University; 2. Co-construction Key Laboratory for Cell Differentiation and Regulation，He’nan Xinxiang 453007, China
  • Received:2012-03-01 Revised:2012-05-07 Online:2013-02-06 Published:2013-02-06

摘要：

目的 了解辅酶Q10（CoQ10）对大鼠再生肝细胞线粒体通透性转换（MPT）的影响。方法 雌性SD大鼠120只，用不同剂量CoQ10灌胃后行部分肝切除术（PH），于术后不同时间取肝组织分离线粒体，分光光度计法测定线粒体悬液的A540nm值，之后再加入钙离子诱导并检测A540nm值。结果 PH后6~48h，高剂量（60mg/kg）CoQ10处理组的再生肝线粒体通透性明显低于对照组，而低剂量（6mg/kg）处理组只在48h明显低于对照组，其他时间均无显著差异。用钙离子诱导后，各组各时间点的线粒体通透性随时间延长而不同程度的增强，但PH后6~48 h的CoQ10处理组的再生肝线粒体通透性稍小于对照组。结论 一定剂量的CoQ10能降低大鼠再生肝线粒体通透性，作用主要体现在肝再生的细胞增殖阶段。

关键词: 辅酶Q10, 线粒体通透性转换, 钙离子, 肝再生, 分光光度计法, 大鼠

Abstract:

Objective  Effects of coenzyme Q10 (CoQ10) on mitochondrial permeability transition(MPT) of regenerating hepatocytes in rats were surveyed in this paper. Methods 120 Sprague-Dawely female rats were randomized to receive three different doses of CoQ10 by gastric lavage daily starting on the day of partial hepatectomy (PH). The rats were killed at different times and the livers were processed for mitochondrial suspension, which was tested with or without Ca 2+ induction by using spectrophotometer. Results During 6-48hours after PH, the mitochondrial permeability of the group treated with high dose（60mg/kg CoQ10）was much lower than that of the control group. The group treated with low dose（6mg/kg CoQ10）was much lower than the control at 48 hours only. However, no statistically significant difference was found among all the groups at 72 hours after PH and later; The mitochondrial permeability of each group was enhanced along with time after the mitochondrial suspension was induced by calcium ion, but the variation of the group treated with CoQ10 was less than the control during 6-48 hours after PH. Conclusion Treating with proper dose of CoQ10 may reduce the mitochondrial permeability of regenerating hepatocytes; the effects mainly take place at the phase of cell proliferation.

Key words: CoQ10, Mitochondria permeability transition, Ca2+, Liver regeneration, Spectrophotometer, Rat