解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (1): 37-40.doi: 10.3969/j.issn.0529-1356.2014.01.007

• 神经生物学 • 上一篇    下一篇

Olig对少突胶质细胞介导脱髓鞘大鼠髓鞘再生的影响

姚宏波1 张萌1 宫学武2 孙丽慧1*   

  1. 1.齐齐哈尔医学院组织学胚胎学教研室，黑龙江 齐齐哈尔 161006; 2.齐齐哈尔医学院第二附属医院眼科， 黑龙江 齐齐哈尔 161006
  • 收稿日期:2012-12-26 修回日期:2013-02-19 出版日期:2014-02-06 发布日期:2014-02-06
  • 通讯作者: 陈志伟 E-mail:24776187@qq.com
  • 基金资助:

    市自然科学基金资助项目

Effects of remyelination of Olig on oligodendrocyte mediated demyelination in the rat

YAO Hong-bo1  ZHANG Meng1  GONG Xue-wu2  SUN Li-hui 1*   

  1. 1.Department of Histology and Embryology, Qiqihar Medical College, Heilongjiang Qiqihar 161006,China;
    2.Department of Ophthalmology，The Second Affiliated Hospital of Qiqihar Medical College, Heilongjiang, Qiqihar 161006, China
  • Received:2012-12-26 Revised:2013-02-19 Online:2014-02-06 Published:2014-02-06
  • Contact: SUN Li-hui E-mail:24776187@qq.com

摘要：

目的 探讨Olig在少突胶质细胞介导的脱髓鞘大鼠髓鞘再生中的作用。方法 40只健康Wistar大鼠，随机分成正常组、模型对照组、模型组、实验组，用形态学观察和免疫组织化学检测Olig1和Olig2的表达。结果 正常组Olig1位于少突胶质细胞的胞质，模型组胞核表达明显增多，实验组Oligl又重新转移至胞质；正常组Olig2表达位于胞核，模型组中有少量表达于胞质。 结论 在Olig1和Olig2同时缺失时，导致全脑不能形成少突胶质细胞，严重影响髓鞘的再生。

关键词: Olig1, Olig2, 少突胶质细胞, 髓鞘再生, 骨髓基质干细胞, 移植, 免疫组织化学, 大鼠

Abstract:

Objective To investigate the role of oligodendrocytes mediated demyelination in the rat about Olig in myelin regeneration. Methods Forty healthy Wistar rats were randomly divided into normal group, model control group, model group and research group. Morphological observation and Immuno- histochemistry detection expression of Olig1 and Olig2 were performed. Results Olig1 was located the cytoplasm of oligodendrocytes in normal group, nuclear expression was increased significantly in model group, and Olig1 was retransferred to the cytoplasm in the research group. Expression of Olig2 was located in the nucleus in the normal group. A few of Olig2 were expressed in the cytoplasm in the model group. Conclusion The brain may not be able to generate oligodendrocytes when Oligl and Olig2 are deficient, which is affect of the myelin regeneration.

Key words: Olig1, Olig2, Oligodendrocyte, Remyelination, Bone marrow stromal cell, Transplantation, Immunohistochemistry, Rat