解剖学报

解剖学报 ›› 2015, Vol. 46 ›› Issue (6): 797-801.doi: 10.16098/j.issn.0529-1356.2015.06.012

• 肿瘤生物学 • 上一篇    下一篇

二氢杨梅素预干预对人肺癌A549细胞放射治疗的敏感性作用

孙大永1 毕红东2 梁鸿鹄1 左彦珍2* 王爱民3 焦海涛4   

  1. 1. 承德市中心医院肿瘤放化疗中心，河北 承德 067000; 2. 承德医学院药理教研室，河北 承德 067000; 3. 承德市中心医院消化科，河北 承德 067000; 4 承德医学院临床技能教研室，河北 承德 067000
  • 收稿日期:2015-06-03 修回日期:2015-08-08 出版日期:2015-12-06 发布日期:2015-12-06
  • 通讯作者: 左彦珍 E-mail:yanzhen926@163.com
  • 基金资助:

    河北省教育厅项目;河北省卫生厅重点课题;承德市科学技术研究项目

Radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro

SUN Da-yong1 BI Hong-dong2 LIANG Hong-hu1 ZUO Yan-zhen 2*WANG Ai-min3 JIAO Hai-tao4   

  1. 1. Department of Tumor Radiation and Chemotherapy Center, Chengde Central Hospital; 2. Department of Pharmacology, Chengde Medical University. Chengde, Hebei 067000, China;3. Department Digestive, Chengde Central Hospital, Hebei Chengde 067000, China;4. Department of Clinical Skill, Chengde Medical University, Hebei Chengde 067000, China

  • Received:2015-06-03 Revised:2015-08-08 Online:2015-12-06 Published:2015-12-06
  • Contact: ZUO Yan-zhen E-mail:yanzhen926@163.com

摘要：

目的 探索二氢杨梅素（DMY）对人肺腺癌A549细胞放射治疗敏感性的调节作用，及新的肺癌放疗增敏剂。方法 采用MTT法和集落形成实验分别寻找二氢杨梅素对A549细胞的短期和长期20%抑制浓度（IC20）作为放疗增敏浓度；流式细胞术周期实验检测二氢杨梅素（IC20）作用不同时间对A549细胞周期时相的改变；集落形成实验检测二氢杨梅素（IC20）预干预24h后再照射对肺癌A549细胞放疗敏感性的影响。结果 MTT和集落形成实验均显示，DMY对A549细胞以浓度依赖形式存在短期和长期的抑制作用，MTT实验时IC20 为 40mg/L，集落形成实验时IC20 为7mg/L；流式细胞术周期实验结果显示，二氢杨梅素作用24h时G2/M期比率增高明显（P<0.05）；集落增敏实验结果显示，DMY预处理24h后对A549细胞的放射治疗并未见到显著增敏作用(P>0.05)。结论 二氢杨梅素体外存在抑制A549细胞增殖的作用，其效应与药物浓度和细胞初始接种密度有关；二氢杨梅素作用24h可以使A549细胞G2/M期比率明显增加，但是单纯通过调整放疗前细胞周期时相并未见到明显的放疗增敏作用。

关键词: 二氢杨梅素, A549细胞, 放射治疗, 敏感性, 流式细胞术, 人

Abstract:

Objectiv To study the radiosensitivity of dihydromyricetin (DMY) on human non-small cell lung cancer A549 cells, and to explore a new drug for lung cancer radiotherapy sensitization.Methods MTT assay and colony formation assay were used to determined the IC20 of dihydromyricetin at different density A549 cells. Flow cytometry (FCM) was used to detect the change of A549 cell cycle phase impacted by DMY of IC20during different time (6, 12, 24, 48hours). Colony formation assay was used to determine the radiosensitivity on A549 cells by dihydromyricetin pre-intervention in vitro. Results The proliferation of A549 cells was inhibited in a dose-dependent manner, MTT assay showed the IC20 was 40mg/L and colony formation assay showed the IC20was 7mg/L. FCM cycle experiment showed that the rate of G2/M phase ratio obviously increased when A549 cells was incubated 24 hours with DMY；A significant sensibilization leading by the effect of radiotherapy sensibilization on A549 cells after DMY pre-intervention 24 hours was not detected by clone formation sensibilization assay.Conclusion The proliferation of A549 cells was inhibited in a dose-dependent manner of dihydromyricetin, and the effect was influenced by cell density. Although the rate of G2/M phase ratio obviously increased when A549 cells were incubated 24 hours with DMY,  there was no significant sensibilization on A549 cells after DMY pre-intervention 24 hours. A further study should be made.

Key words: Dihydromyricetin, A549 cell, Radiosensitivity, Sensibilization, Flow cytometry, Human