解剖学报

解剖学报 ›› 2015, Vol. 46 ›› Issue (4): 488-494.doi: 10.16098/j.issn.0529-1356.2015.04.008

• 细胞和分子生物学 • 上一篇    下一篇

新生大鼠多器官成纤维细胞nanog蛋白的表达差异

鲁召辉1 常玉巧1 郭志坤1* 赵国安2 李琼1 李辞霞1   

  1. 1.新乡医学院河南省医用组织再生重点实验室; 2.第三附属医院心脏内科，河南 新乡 453003
  • 收稿日期:2014-12-23 修回日期:2015-04-06 出版日期:2015-08-06 发布日期:2015-08-06
  • 通讯作者: 郭志坤 E-mail:gzk@xxmu.edu.cn

Expression of nanog protein in fibroblasts from multiple organs of the neonatal rat

LU Zhao-hui1 CHANG Yu-qiao1 GUO Zhi-kun 1* ZHAO Guo-an2 LI Qiong1 LI Ci-xia1   

  1. 1. He’nan Key Laboratory of Medical Tissue Regeneration; 2. Internal Medicin-Cardiovascular Department, Third Affilated Hospital, Xinxiang Medical University, He’nan Xinxiang 453003, China
  • Received:2014-12-23 Revised:2015-04-06 Online:2015-08-06 Published:2015-08-06
  • Contact: GUO Zhi-kun E-mail:gzk@xxmu.edu.cn

摘要：

目的 探讨新生大鼠心、肝、脾、肺、肾和皮肤中成纤维细胞nanog蛋白表达差异及其意义。方法 用免疫组织化学、免疫荧光和体外细胞培养技术，对成纤维细胞进行nanog蛋白和波形蛋白（vimentin）标记，检测并比较新生大鼠不同器官中成纤维细胞nanog蛋白和vimentin表达及共表达特征。 结果 新生大鼠心、肝、脾、肺、肾和皮肤6种器官中均有nanog蛋白和vimentin表达，在每个器官中均有少数成纤维细胞共表达这两种蛋白，共表达率分别为心（10.07±0.77）%、肝（9.97±3.70）%、脾（11.23±2.98）%、肺（15.07±2.87）%、肾（11.75±2.76）%、皮肤（16.14±1.28）%，其中皮肤和肺的共表达率高于心、肝、脾、肾（P<0.05）。 结论 共表达nanog蛋白和vimentin的成纤维细胞可能更具有干细胞样特性；新生大鼠皮肤和肺的成纤维细胞可能更具有组织损伤修复潜能。

关键词: nanog蛋白, 波形蛋白, 成纤维细胞, 免疫组织化学, 免疫荧光, 新生大鼠

Abstract:

Objective To investigate different expression and its significance of nanog protein in multiple organs fibroblasts of neonatal rats, including heart, liver, spleen, lung, kidney and skin. Methods The expresssion and co-expression of nanog and vimentin in fibroblasts were detected in multiple organs by immunohistochemistry and immunofluorescence. Fibroblasts in multiple organs cultured in vitrowere labeled with nanog and vimentin by immunofluorescence. Results Nanog and vimentin were detected in six organs in neonatal rats respectively. Co-expression rates of these two proteins were（10.07±0.77）% in heart,（9.97±3.70）% in liver,（11.23±2.98）% in spleen,（15.07±2.87）% in lung,（11.75±2.76）% in kidney and（16.14±1.28）% in skin. So co-expression rates of skin and lung were higher than that of other four organs（P<0.05）. Conclusion Fibroblasts in multiple organs which can express nanog may have stem cell-like characters and fibroblasts in skin and lung of neonatal rats have more potential for organ repair.

Key words: Nanog, Vimentin, Fibroblast, Immunohistochemistry, Immunofluorescence, Neonatal rat