体外诱导骨髓间充质干细胞成肝(样)细胞的研究进展

doi:10.16098/j.issn.0529-1356.2015.05.023

摘要/Abstract

摘要：

肝病已成为世界上发病率和死亡率最高的疾病之一。目前有望治疗晚期重症肝病的方法有原位肝移植、肝细胞移植、生物人工肝等，但这些方法都面临着肝或肝细胞源紧缺的问题。如何能够获得体外增殖的、可用于移植疗法的肝细胞资源成为研究的要点。骨髓间充质干细胞（BMSCs）既具有自我更新的能力，也具有在合适的微环境里多系横向分化的潜能，在体外可以被诱导分化为肝(样)细胞，理论上可提供丰富的肝细胞，BMSCs成为了细胞治疗的研究热门。我们简要综述了体外诱导BMSCs向肝样细胞（HLCs）分化的研究成果和最新进展，总结了各种诱导方法的优势以及存在的问题，并对该研究领域进行了小结与展望。

关键词: 骨骨髓间充质干细胞, 诱导, 分化, 肝样细胞

Abstract:

Liver diseases have become one of the most important causes of morbidity and mortality in the world. Therapeutic methods such as orthotopic liver transplantation, hepatocyte transplantation, bioartificial liver etc are considered to treat the advanced liver disease. However, the lack of liver donor in liver transplantation and the shortage of cells sources in cytotherapy are the major obstacles for these treatment methods. Exploring new origins of extra-hepatic cells is needed. Bone marrow mesenchymal stem cells (BMSCs), characterized as multiple differentiation, rapid self-renewal, and easy to be cultured and proliferated, have been considered as the most promising source of cells. A number of recent studies have shown that BMSCs can be differentiated into hepatocytes like cells (HLCs) in vitroandin vivo. This paper reviews the up-to-date achievements and progresses in the field of isolation, characterization and induction of BMSCs, summarizes the superiority and existence question of each kind of induction condition, and finally makes a summary and expectation for this research field.

Key words: Bone marrow mesenchymal stem cell, Induction, Differentiation, Hepatocyte-like cell

代克强丁一樊晋宇徐存拴*. 体外诱导骨髓间充质干细胞成肝(样)细胞的研究进展[J]. 解剖学报, 2015, 46(5): 704-709.

DAI Ke-qiang DING Yi FAN Jin-yu XU Cun-shuan*. Advance in the study of bone marrow mesenchymal stem cell differentiation into hepatocyte in vitro[J]. AAS, 2015, 46(5): 704-709.

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