解剖学报

解剖学报 ›› 2015, Vol. 46 ›› Issue (5): 591-595.doi: 10.16098/j.issn.0529-1356.2015.05.003

• 神经生物学 • 上一篇    下一篇

生长抑素阳性神经元在大鼠纹状体的形态分布

张文君 俞明远 王利利 滕可导 马云飞*   

  1. 中国农业大学动物医学院，北京 100193
  • 收稿日期:2015-02-09 修回日期:2015-05-08 出版日期:2015-10-06 发布日期:2015-10-06
  • 通讯作者: 马云飞 E-mail:yunfeima@cau.edu.cn
  • 基金资助:

    教育部中央高校基本科研业务费专项资金

Distribution and morphological features of the somatostatin positive neurons in the rat striatum

ZHANG Wen-jun YU Ming-yuan WANG Li-li TENG Ke-dao MA Yun-fei*   

  1. Animal Medical School of China Agricultural University, Beijing 100193, China
  • Received:2015-02-09 Revised:2015-05-08 Online:2015-10-06 Published:2015-10-06
  • Contact: MA Yun-fei E-mail:yunfeima@cau.edu.cn
  • Supported by:

    Chinese Universities Scientific-fund from the Ministry of Education of China

摘要：

目的 探讨成年大鼠纹状体内生长抑素（SOM）阳性神经元的形态分布。方法 选取6只SD大鼠，应用免疫细胞化学染色ABC法观察脑纹状体SOM神经元的分布特征。 结果 在大鼠纹状体内SOM免疫反应阳性神经元呈棕黄色，为强阳性或中等阳性染色，免疫反应阳性物质主要存在于胞质和突起。SOM阳性神经元在纹状体呈散在分布，胞体中等大小［（111.81±21.42）μm2］，多为卵圆形、椭圆型或多边形。背侧［（113.52±22.36）μm2］SOM阳性神经元胞体面积大于腹侧［（110.14±20.59）μm2］，外侧［（126.23±23.45）μm2］大于内侧［（97.26±23.63）μm2］。腹内侧区SOM阳性细胞数量百分比最多［（28.34±3.20）％］，其次是背内侧区［（27.64±3.52）％］和腹外侧区［（2498±317）％］，背外侧区最少［（1904±312）％］。 结论 SOM神经元在纹状体结构各区分布的大小及数量不完全相同。

关键词: 生长抑素, 纹状体, 免疫组织化学, 大鼠

Abstract:

Objective To explore the distribution of somatostatin (SOM) in the striatum of the adult rat. Methods SOM expressions in the six adult rats were localized using immunohistochemistry (ABC method). Results In the rat striatum, SOM positive neurons, yellow brown in color, had strong or moderate immunoreactivity and were mainly distributed in cytoplasm and cytoplasmic processes. Somas were in medium size（111.81±21.42）μm2, and oval orpolygonal in shape. The dosal SOM immunoreactive neurons（113.52±22.36）μm2 were larger than the ventral (110.14±20.59)μm2. The outside SOM immunoreactive neurons（126.23±23.45）μm2 were larger than the inside（97.26±23.63）μm2. SOM immunoreactive neurons were distributed in the ventral medial side（28.34±3.20）％, dorsal-medial region (27.64±3.52)％, ventral-lateral region（24.98±3.17）％, and dorsal-lateral region（19.04±3.12）％. Conclusion The number and size of SOM immunoreactive neurons differ, unveiling the role of SOM in the neural circuits of striatum and providing some morphological basis.

Key words: Somatostatin, Striatum, Immunohistochemistry, Rat