索拉非尼对大鼠佐剂性关节炎的抑制作用

doi:10.16098/j.issn.0529-1356.2017.02.012

摘要/Abstract

摘要：

目的探讨索拉非尼对大鼠佐剂性关节炎(AA)的抑制作用。方法 36只雄性SD大鼠均分为6组，除正常组外，其余各组大鼠均制备AA模型。足容积法检测AA大鼠继发侧足爪容积；流式细胞术检测外周血CD4⁺及CD8⁺T细胞亚群的变化；免疫组织化学链霉卵白素-过氧化物酶法(SP)检测滑膜组织微血管密度(MVD)的改变。结果与模型组相比，索拉非尼组大鼠足爪容积下降，滑膜组织MVD减小，其中索拉非尼(20、40mg/kg)组可使外周血CD4⁺T细胞比例降低，CD8⁺T细胞比例增加，差异均有统计学意义(P<0.05)。结论索拉非尼具有抑制大鼠AA效应，该作用可能与索拉非尼引起AA大鼠外周血CD4⁺, CD8⁺T细胞亚群的偏移以及降低滑膜组织MVD有关。

关键词: 佐剂性关节炎, 索拉非尼, T细胞亚群, 微血管密度, 免疫组织化学SP法, 流式细胞术, 大鼠

Abstract:

Objective To observe the inhibition effect of sorafenib on adjuvant arthritis in rats. Methods A total of 36 male SPF SD rats were divided into 6 groups and 0.1ml of the complete Freund’s adjuvant was subcutaneously injected into the left hind paw except the normal group. The volume of rat hind paw was measured. The CD4⁺ and CD8⁺T cell subsets of the peripheral blood were analyzed by flow cytometry. The microvessel density of synovial tissues was determined by immunohistochemical chain mildew avidin peroxidase enzymatic (SP) method. Results Compared with model group, the rats of sorafenib groups alleviated the volume of rat hind paw and reduced microvessel density. Sorafenib (20, 40mg/kg) groups decreased the percentage of CD4⁺T cell and increased the percentage of CD8⁺T cell at the same time. All the values were statistically significant(P<0.05). Conclusion Sorafenib can ameliorate adjuvant arthritis in rats, which effect may be related to sorafenib causing CD4+ and CD8+T cell subset of peripheral blood deviation and reducing microvessel density of synovial tissues.

Key words: Adjuvant arthritis, Sorafenib, T cell subset, Microvessel density, Immunohistochemical SP method, Flow cytometry, Rat

刘菲龚永芳王真珍张晓明邓雪飞焦轶黄学应. 索拉非尼对大鼠佐剂性关节炎的抑制作用[J]. 解剖学报, 2017, 48(2): 187-192.

LIU Fei GONG Yong-fang WANG Zhen-zhen ZHANG Xiao-ming DENG Xue-fei JIAO Yi HUANG Xue-ying. Inhibition effect of sorafenib on adjuvant arthritis in rats[J]. Acta Anatomica Sinica, 2017, 48(2): 187-192.

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