关键词: 心肌样本, 特殊染色, 免疫组织化学, 透射电子显微镜, 人

Abstract:

Objective To investigate the use of morphology inspection methods in myocardial diseases, and to evaluate their advantages and disadvantages. Methods Myocardial tissues were staining by phosphotungstic aci hematoxylin(PTAH)，Masson，elastic fiber tissue (ET)+Van Gienson(VG)， Congo red，Schiff periodic acid shiff(PAS)，and Gram staining methods. Immunehistochemical method, and transmission microscopy had also been applied to study the myocardial tissue. Results HE staining differentiation of myocardial tissue was not well; Masson trichromatic staining differentiation of myocardial cells and collagen fibers had clear distinction; ET + VG staining method was able to distinguish among elastic fibers,collagen fibers, fibers and amyloid; Congo red stain was mainly used in the detection of amyloidosis, the PAS reaction assisted in the diagnosis of glycogen storage disease. Immunohistochemical technology was an useful tool in disease model study and diagnosis of the cardiac disease. Electron microscopic observation of the ultrastructure was an important means of etiological diagnosis. Conclusion For myocardial tissue samples, a combination of morphology methods of the histology, subcellular and molecular can get a better observation.

Key words: Cardiac sample, Special dyeing, Immunohistochemistry, Transmission microscopy, Human