脑内移植RMNE6细胞治疗缺血性脑损伤的效果

doi:10.16098/j.issn.0529-1356.2017.06.001

解剖学报 ›› 2017, Vol. 48 ›› Issue (6): 635-641.doi: 10.16098/j.issn.0529-1356.2017.06.001

• 神经生物学 • 下一篇

脑内移植RMNE6细胞治疗缺血性脑损伤的效果

武赟^1,2*翟晓艳¹杨李旺¹徐群渊²

1. 山西中医药大学基础医学院解剖学教研室，山西晋中 030619；2.首都医科大学神经生物学系，北京 100069

收稿日期:2016-12-30 修回日期:2017-02-24 出版日期:2017-12-06 发布日期:2017-12-06
通讯作者: 武赟 E-mail:wu_yun19741981@163.com
基金资助:
促血管神经发生水凝胶载体与神经干细胞联合移植治疗小鼠脑梗塞模型的实验研究

Effects of intracerebral transplantation of RMNE6 on the ischemic injury of the brain

WU Yun ^1,2* ZHAI Xiao-yan¹ YANG Li-wang¹ XU Qun-yuan²

1.Department of Anatomy, Basic Medical College, Shanxi University of Chinese Medicine，Shanxi Jinzhong 030619, China；2.Department of Neurobiology, Capital Medical University, Beijing 100069, Chnia

Received:2016-12-30 Revised:2017-02-24 Online:2017-12-06 Published:2017-12-06
Contact: WU Yun E-mail:wu_yun19741981@163.com

摘要/Abstract

摘要：

目的为细胞替代治疗缺血性脑损伤选择合适的干细胞。方法将RMNE6细胞植入正常脑和缺血模型大鼠脑(8只)内，通过免疫荧光染色、行为学实验、影像学等方法观察其在脑内的状况，与安慰剂组（8只）及成纤维细胞移植组（8只）比较，观察其对模型动物梗塞体积的影响和模型动物行为学的改善。结果细胞移植后，RMNE6细胞在正常脑和缺血脑内可以存活，但移植有RMNE6细胞的缺血脑内出现肿瘤。细胞移植与否对梗死体积没有影响。细胞移植后，模型动物的行为学也未见明显改善。结论从安全性的角度考虑，RMNE6细胞不适合通过脑内移植治疗缺血性脑损伤，RMNE6细胞还需要改造。

关键词: RMNE6细胞, 细胞移植, 脑缺血, 免疫荧光, 磁共振成像, 大鼠

Abstract:

Objective To select the suitable stem cells for cell replacement in stroke therapy. Methods The RMNE6 cells were transplanted into the normal brain and injured brain of the stroke model of rats (n=8). The fates of the RMNE6 cells in the normal brain and the injured brain, the effects on the ischemic volume and the improvements on the behavior of the rats were observed by immunofluorescence staining, behaviour testing and magnetic resonance imaging, compared with the placebo group (n=8) and the fibroblast group (n=8). Results After transplantation, the RMNE6 cells were able to survive in the normal and ischemic brain area. There was tumourigenesis in the ischemic brain transplanted with the RMNE6 cells. The cell transplantation had no effects on the size of the ischemic volume. The behavior of the model animals had no significiant improvement. Conclusion As far as the safety is concerned, the RMNE6 cells are not suitable for cell replacement in the stroke therapy and still need to be modified.

Key words: RMNE6 cell, Cell transplantation, Brain ischemia, Immunofluorescence, Magnetic resonance imaging, Rat

武赟翟晓艳杨李旺徐群渊. 脑内移植RMNE6细胞治疗缺血性脑损伤的效果[J]. 解剖学报, 2017, 48(6): 635-641.

WU Yun ZHAI Xiao-yan YANG Li-wang XU Qun-yuan. Effects of intracerebral transplantation of RMNE6 on the ischemic injury of the brain[J]. Acta Anatomica Sinica, 2017, 48(6): 635-641.

参考文献

［1］Nishino H, Borlongan CV.Restoration of function by neural transplantation in the ischemic brain ［J］. Prog Brain Res, 2000, 127:461-476.

［2］Savitz SI, Rosenbaum DM, Dinsmore JH, et al. Cell transplantation for stroke ［J］. Ann Neurol, 2002, 52(3):266-275.

［3］Wang B, Zou X, Zhang H, et al. Establishment of an immortalized GABAergic neuronal progenitor cell line from embryonic ventral mesencephalon in the rat ［J］. Brain Res, 2008, 1210(19):63-75.

［4］Wu Y, Wu J, Ju R, et al. Comparison of intracerebral transplantation effects of different stem cells on rodent stroke models ［J］. Cell Biochem Funct, 2015, 33(4):174-182.

［5］Neumann-Haefelin T, Kastrup A, de Crespigny A, et al.Serial MRI after transient focal cerebral ischemia in rats: dynamics of tissue injury, blood-brain barrier damage, and edema formation ［J］. Stroke, 2000, 31(8):1965-1973.

［6］Brabb T, von Dassow P, Ordonez N, et al. In situ tolerance within the central nervous system as a mechanism for preventing autoimmunity ［J］. J Exp Med, 2000, 192(6):871-880.［7］Widner H, Brundin P. Immunological aspects of grafting in the mammalian central nervous system. A speculative synthesis ［J］. Brain Res Rev, 1988, 472(3):287-324.

［8］Hickey WH. Basic principles of immunological surveillance of the normal central nervous system ［J］. Glia, 2001,36(2):118-124.

［9］Ohtsuka M, Inoko H, Kulski JK, et al. Major histocompatibility complex (Mhc) class Ib gene duplications, organization and expression patterns in mouse strain C57BL/6 ［J］. BMC Genomics, 2008, 17(9):178.

［10］Shatz CJ. MHC class I: an unexpected role in neuronal plasticity ［J］. Neuron, 2009，64(1):40-45.

［11］Chen J, Li Y, Wang L, et al. Therapeutic benefit of intracerebral transplantation of bone marrow stromal cells after cerebral ischemia in rats ［J］. J Neurol Sci, 2001, 189(1-2):49-57.

［12］Li Y, Chopp M, Chen J, et al. Intrastriatal transplantation of bone marrow nonhematopoietic cells improves functional recovery after stroke in adult mice ［J］. J Cereb Blood Flow Metab, 2000, 20(9):1311-1319.

［13］Takahashi K, Yasuhara T, Shingo T, et al. Embryonic neural stem cells transplanted in middle cerebral artery occlusion model of rats demonstrated potent therapeutic effects, compared to adult neural stem cells ［J］. Brain Res, 2008, 1234:172-182.

[1]	谢惠兰方芳林毅. Chir99021调控Wnt/β-catenin 信号通路抑制大鼠牙髓干细胞成骨诱导分化的机制[J]. 解剖学报, 2024, 55(1): 67-72.
[2]	魏茜茜李超红赵晨露唐家萍刘玉珍. 大鼠颈上神经节中Ⅰ组代谢型谷氨酸受体表达及慢性间歇性低氧对其的影响 [J]. 解剖学报, 2024, 55(1): 3-9.
[3]	苏芃王兴仪梁景岩熊天庆. 一种低密度及高纯度胎鼠原代海马神经元培养方法的优化及鉴定[J]. 解剖学报, 2024, 55(1): 113-119.
[4]	郝永明郭磊周程辉裴汉军李莉赵哲 . 改良腹主动脉缩窄法建立心肌肥厚模型[J]. 解剖学报, 2024, 55(1): 120-124.
[5]	许亚平余悦欣陈金福王柯柯郭志坤 . 高龄大鼠心室肌间质弹性纤维和胶原纤维的结构分布特征及其机制 [J]. 解剖学报, 2023, 54(6): 716-721.
[6]	刘丽平朱梦妮徐慧 . 白细胞介素6对脂多糖诱导所致类子痫前期大鼠有核红细胞的影响 [J]. 解剖学报, 2023, 54(6): 722-729.
[7]	赵卫明李晓余国营王改平常翠芳 . 丝氨酸肽酶抑制因子Kazal型1对肝癌细胞增殖的影响及其机制 [J]. 解剖学报, 2023, 54(6): 695-702.
[8]	王文娟丁雨桐苏昊任捷孙艳荣王涵菲陆嘉莉张林倩白钰秦丽华. 低雌激素状态下大鼠海马及纹状体Nogo-A的表达变化[J]. 解剖学报, 2023, 54(6): 620-627.
[9]	梁盼盼禹爱梅杜静寇文辉王欢欢宋爱霞. 基于c-Jun氨基末端激酶/p38丝裂原活化蛋白激酶信号通路探讨阿魏酸钠对偏头痛大鼠炎症反应的抑制作用[J]. 解剖学报, 2023, 54(6): 652-659.
[10]	姚素华鄢骏谢芳林春华黄争春 . 奥氮平介导环磷酸腺苷反应元件结合蛋白/脑源性神经营养因子/酪氨酸蛋白激酶受体B通路对精神分裂模型大鼠的神经修复作用 [J]. 解剖学报, 2023, 54(6): 660-667.
[11]	徐颖刘斌郑兴何宗钊. 补体C3a受体在盲肠结扎穿孔致脓毒症大鼠认知障碍中的作用及机制 [J]. 解剖学报, 2023, 54(6): 668-675.
[12]	刘哲川李坤马帅男孟家琪王艳芹. 利拉鲁肽对百草枯诱导的小鼠帕金森病模型炎症及线粒体融合/分裂的影响 [J]. 解剖学报, 2023, 54(6): 676-681.
[13]	李霞朱世杰唐中生罗亚非范瑞娟谢高宇寇云芳陆莹 . 电针智三针调节酪氨酸激酶受体的配体通路改善血管性痴呆的作用机制[J]. 解剖学报, 2023, 54(6): 689-694.
[14]	孙娟陈敬威杨艺林涛牟雅琳赵秀丽曾国熙张艳 . 缺氧预处理通过缺氧诱导因子-1α/基质细胞衍生因子-1通路对大鼠血液学相关指标的影响[J]. 解剖学报, 2023, 54(5): 505-511.
[15]	张亚清吉维忠王玲. N-甲基-D-天冬氨酸受体/MAPK/环磷腺苷效应元件结合蛋白通路在前扣带皮层介导痛相关情绪[J]. 解剖学报, 2023, 54(5): 512-520.

脑内移植RMNE6细胞治疗缺血性脑损伤的效果

Effects of intracerebral transplantation of RMNE6 on the ischemic injury of the brain

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价